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Insulin Receptor 抗体 (Beta Chain)

Name: Insulin Receptor 抗体 (Beta Chain)
SKU: ABIN126821
Availability: OutOfStock

INSR 适用: 人, 小鼠, 大鼠, 犬 WB, EIA 宿主: 小鼠 Monoclonal 11B6 unconjugated

Western Blotting (WB) image for anti-Insulin Receptor (INSR) (Beta Chain) antibody (ABIN126821) (Insulin Receptor 抗体 (Beta Chain))

1 image

产品编号 ABIN126821

发货至: 中国

Datasheet as PDF

抗原 See all Insulin Receptor (INSR) 抗体

Insulin Receptor (INSR)
抗原表位
16

15

13

12

9

8

8

7

6

6

5

5

5

4

4

4

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Beta Chain
适用
167

95

80

15

14

9

8

7

6

5

4

3

2

2

1

1

1

1

1

1

1
人, 小鼠, 大鼠, 犬
宿主
165

22

3

1
小鼠
Compatible Secondaries
克隆类型
158

33
单克隆
标记
116

16

16

7

6

5

3

3

3

3

3

3

1

1

1

1

1

1

1
This Insulin Receptor antibody is un-conjugated
应用范围
139

82

52

28

16

15

15

14

13

13

8

5

4

4

3

2

1

1

1

1
Western Blotting (WB), Enzyme Immunoassay (EIA)

特异性

This antibody specifically recognizes the C-terminus of Insulin receptor (phosphorylation-independent).

产品特性

Synonyms: Insulin Receptor

纯化方法

Subsequent thiophilic adsorption and size exclusion chromatography

组件

incl. positive Control

免疫原

Synthetic peptide conjugated to KLH

克隆位点

11B6

亚型

IgG1

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anti-Insulin Receptor (INSR) (AA 1024-1298) antibody
INSR 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor (INSR) (AA 1027-1364) antibody
INSR 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor (INSR) (AA 1101-1372) antibody
INSR 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor (INSR) (C-Term) antibody
INSR 适用: 人, 小鼠, 大鼠, 猴, 犬, 马, Cow, 豚鼠, Pig, 兔, 小鸡, 绵羊, Hamster, 山羊, Avian, Cat, 驴 WB 宿主: 山羊 Polyclonal unconjugated

anti-Insulin Receptor (INSR) (C-Term) antibody
KO Validated INSR 适用: 人 WB, ICC, IHC (p), IF 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor (INSR) (AA 51-150) antibody
INSR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor (INSR) (AA 1325-1358) antibody
INSR 适用: 小鼠 WB 宿主: 兔 Polyclonal RB50340 unconjugated

anti-Insulin Receptor (INSR) (AA 1023-1298) antibody
INSR 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor (INSR) (AA 27-57), (N-Term) antibody
INSR 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB1421 unconjugated

应用备注

Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from untreated HepG2 cells. ELISA: 0.1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

实验流程

Positive Control Cell LysateFormulation: Lyophilized cell lysate from serum starved HepG2 cells. Recostitution: Reconstitute by addition of 200 µl H2O. After complete solubilization add200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90 °C for 5 min. Aliquote and store frozen. Avoid repeated freezing and thawing. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80. 000 cells. Use 20µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation.

限制

仅限研究用
溶解方式

Restore with 1 mL H2O (15 min, RT).

缓冲液

1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -20 to -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
Shelf life: one year from despatch.

有效期

12 months
抗原

Insulin Receptor (INSR)

别名

CD220 / INSR (INSR 产品)

别名

CD220 antibody, HHF5 antibody, 4932439J01Rik antibody, D630014A15Rik antibody, IR antibody, IR-A antibody, IR-B antibody, 18402 antibody, CG18402 antibody, DIHR antibody, DILR antibody, DIR antibody, DIRH antibody, DIRbeta antibody, DInR antibody, DInr antibody, Dir-a antibody, Dir-b antibody, Dmel\\CG18402 antibody, INR antibody, INS antibody, Inr antibody, Inr-alpha antibody, Inr-beta antibody, InsR antibody, dINR antibody, dIR antibody, dIRH antibody, dInR antibody, dInr antibody, dInsR antibody, dinr antibody, dir antibody, er10 antibody, inr antibody, insulin/insulin-like growth factor receptor antibody, l(3)05545 antibody, l(3)93Dj antibody, l(3)er10 antibody, lnR antibody, ir-A antibody, CTK-1 antibody, ir antibody, INSR antibody, NV14476 antibody, cd220 antibody, hhf5 antibody, insulin receptor antibody, Insulin-like receptor antibody, insulin receptor L homeolog antibody, INSR antibody, Insr antibody, InR antibody, LOC100122567 antibody, LOC100451802 antibody, insr.L antibody

背景

The insulin receptor (InsR) is a heterodimeric receptor tyrosine kinase with an extracellular alpha-chain, a transmembrane domain and an intracellular beta-chain. The insulin receptor is activated upon binding of the peptide hormone insulin, leading to autophosphorylation of tyrosine residues 1146, 1150, and 1151 in the activation loop of the beta-chain. Additional phosphorylation sites such as tyrosine residues 960, 1316, and 1322 regulate the assembly of signal transduction complexes.Synonyms: Insulin Receptor

基因ID

3643

UniProt

P06213

途径

NF-kappaB Signaling, RTK signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity