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Insulin Receptor 抗体 (Beta Chain)

Name: Insulin Receptor 抗体 (Beta Chain)
SKU: ABIN126821
Availability: OutOfStock

This anti-Insulin Receptor antibody is a 小鼠单克隆 antibody detecting Insulin Receptor in WB 和 EIA. Suitable for 人, 小鼠, 大鼠和犬.

Western Blotting (WB) image for anti-Insulin Receptor (INSR) (Beta Chain) antibody (ABIN126821) (Insulin Receptor 抗体 (Beta Chain))

1 image

产品编号 ABIN126821

发货至: 中国

Quick Overview for Insulin Receptor 抗体 (Beta Chain) (ABIN126821)

抗原

See all Insulin Receptor (INSR) 抗体

Insulin Receptor (INSR)

适用

人, 小鼠, 大鼠, 犬

宿主

小鼠

克隆类型

单克隆

标记

This Insulin Receptor antibody is un-conjugated

应用范围

Western Blotting (WB), Enzyme Immunoassay (EIA)

克隆位点

11B6

Discover our top product INSR Primary Antibody

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Beta Chain

特异性

This antibody specifically recognizes the C-terminus of Insulin receptor (phosphorylation-independent).

产品特性

Synonyms: Insulin Receptor

纯化方法

Subsequent thiophilic adsorption and size exclusion chromatography

组件

incl. positive Control

免疫原

Synthetic peptide conjugated to KLH

亚型

IgG1
anti-Insulin Receptor (INSR) antibody
INSR 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C7 unconjugated

anti-Insulin Receptor (INSR) (AA 1024-1298) antibody
INSR 适用: 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor (INSR) (AA 1027-1364) antibody
INSR 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor (INSR) (AA 1101-1372) antibody
INSR 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor (INSR) (C-Term) antibody
INSR 适用: 人, 小鼠, 大鼠, 犬, 猴, 马, Cow, 豚鼠, 兔, Pig, 小鸡, 绵羊, Hamster, Avian, Cat, 驴, 山羊 WB 宿主: 山羊 Polyclonal unconjugated

anti-Insulin Receptor (INSR) (C-Term) antibody
KO Validated INSR 适用: 人 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor (INSR) (AA 51-150) antibody
INSR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor (INSR) (AA 1325-1358) antibody
INSR 适用: 小鼠 WB 宿主: 兔 Polyclonal RB50340 unconjugated

anti-Insulin Receptor (INSR) (AA 1023-1298) antibody
INSR 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Insulin Receptor (INSR) (AA 27-57), (N-Term) antibody
INSR 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB1421 unconjugated

应用备注

Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from untreated HepG2 cells. ELISA: 0.1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

实验流程

Positive Control Cell LysateFormulation: Lyophilized cell lysate from serum starved HepG2 cells. Recostitution: Reconstitute by addition of 200 µl H2O. After complete solubilization add200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90 °C for 5 min. Aliquote and store frozen. Avoid repeated freezing and thawing. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80. 000 cells. Use 20µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation.

限制

仅限研究用
溶解方式

Restore with 1 mL H2O (15 min, RT).

缓冲液

1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -20 to -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
Shelf life: one year from despatch.

有效期

12 months
抗原

Insulin Receptor (INSR)

别名

CD220 / INSR

背景

The insulin receptor (InsR) is a heterodimeric receptor tyrosine kinase with an extracellular alpha-chain, a transmembrane domain and an intracellular beta-chain. The insulin receptor is activated upon binding of the peptide hormone insulin, leading to autophosphorylation of tyrosine residues 1146, 1150, and 1151 in the activation loop of the beta-chain. Additional phosphorylation sites such as tyrosine residues 960, 1316, and 1322 regulate the assembly of signal transduction complexes.Synonyms: Insulin Receptor

基因ID

3643

UniProt

P06213

途径

NF-kappaB Signaling, RTK signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity