MAPK7 抗体 (N-Term)
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Quick Overview for MAPK7 抗体 (N-Term) (ABIN126797)
抗原
See all MAPK7 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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特异性
- This antibody recognizes MAP kinase 7 (erk5) at 92 kDa in Western-blot applications.
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产品特性
- Synonyms: Mitogen-activated protein kinase 7, Extracellular signal-regulated kinase 5, ERK4, Big MAPkinase 1, MAPK7
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纯化方法
- Size Exclusion Chromatography
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组件
- incl. positive Control
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免疫原
- Peptide conjugated to hemocyanin. AA Sequence: N-Terminus
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亚型
- IgG1
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应用备注
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Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive control: Cell lysate from untreated SKOV-3 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 1 mL H2O (15 min, RT).
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缓冲液
- 1 mLPBS containing 0.09 % Sodium Azide, PEG and Sucrose
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C/-80 °C
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储存方法
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch. -
有效期
- 12 months
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- MAPK7 (Mitogen-Activated Protein Kinase 7 (MAPK7))
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别名
- MAPK7 / ERK5
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背景
- Erk5 (also known as MAPK7 or big mitogen activated kinase 1, BMK1) belongs to the Ser/Thr protein kinase family. It contains a T E Y motif in the activation loop similar to Erk1/2. Extracellular signals, including receptor tyrosine kinases and G-protein-coupled receptors as well as osmotic and oxdative stress lead to Erk5 activation by MEK5. The Erk5 pathway plays an important role in cellular proliferation, differentiation and survival.Synonyms: Big MAP kinase 1, ERK4, Extracellular signal-regulated kinase 5, MAPK7, Mitogen-activated protein kinase 7
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基因ID
- 5598
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UniProt
- Q13164
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途径
- MAPK Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, cAMP Metabolic Process, Toll-Like Receptors Cascades, Negative Regulation of intrinsic apoptotic Signaling
抗原
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