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ERBB3 抗体 (Cytoplasmic Domain)

Name: ERBB3 抗体 (Cytoplasmic Domain)
SKU: ABIN126794
Availability: OutOfStock

ERBB3 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 5A12 unconjugated

Western Blotting (WB) image for anti-Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (AA 1250-1270), (Cytoplasmic Domain) antibody (ABIN126794) (ERBB3 抗体 (Cytoplasmic Domain))

1 image

产品编号 ABIN126794

发货至: 中国

Datasheet as PDF

抗原 See all ERBB3 抗体

ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))
抗原表位
29

26

21

15

15

12

9

8

7

7

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 1250-1270, Cytoplasmic Domain
适用
182

128

113

17

6

4

3

3

2

2

2

1

1

1
人
宿主
189

27

1

1
小鼠
Compatible Secondaries
克隆类型
186

32
单克隆
标记
113

13

10

10

10

10

10

10

5

5

4

4

4

4

4

1

1
This ERBB3 antibody is un-conjugated
应用范围
102

67

53

53

50

31

28

20

19

16

8

3

3

3

2

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

特异性

This antibody recognizes erbB3 phosphorylation status independent at 185 kDa in Western blot.

产品特性

Synonyms: ERBB-3, c-erbB-3, HER-3, Receptor tyrosine-protein kinase erbB-3, Tyrosine kinase-type cellsurface receptor HER3, Proto-oncogene-like protein c-ErbB-3

纯化方法

Subsequent Ultrafiltration and Size Exclusion Chromatography.

组件

incl. positive Control

免疫原

Peptide conjugated to hemocyanin (aa 1250-1270).

克隆位点

5A12

亚型

IgG1

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anti-Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (AA 24-55), (N-Term) antibody
ERBB3 适用: 人, 小鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB01467 unconjugated

anti-Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (pTyr1289) antibody
ERBB3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (AA 1-130) antibody
ERBB3 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2E9 unconjugated

anti-Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (Tyr1328) antibody
ERBB3 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (C-Term) antibody
ERBB3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (AA 24-55) antibody
ERBB3 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (AA 661-760) antibody
ERBB3 适用: 人, 小鼠, 大鼠 ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (pTyr1328) antibody
ERBB3 适用: 人, 大鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) antibody
ERBB3 适用: 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated

应用备注

Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20. Positive Control: Cell lysate from untreaed SW620 cells. (See Protocols)Immunohistochemistry on Paraffin Sections. Immunocytochemistry: (1-10 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

实验流程

Positive Control: Cell lysate from untreated SW620 cellsFormulation: Lyophilized cell lysate from serum starved SW 620 cellsReconstitution: Reconstitute by addition of 200 µl H2O. After complete solubilization add200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90 °C for 5 min. Aliquote and store frozen. Avoid repeated freeze/thaw cycles.

限制

仅限研究用
溶解方式

Restore with 1 mL H2O (15 min, RT).

缓冲液

1 mLPBS, 0.09 % Sodium Azide, PEG and Sucrose

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C/-80 °C

储存方法

Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

有效期

12 months
抗原

ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

别名

ERBB3 / HER3 (ERBB3 产品)

别名

ErbB-3 antibody, HER3 antibody, LCCS2 antibody, MDA-BF-1 antibody, c-erbB-3 antibody, c-erbB3 antibody, erbB3-S antibody, p180-ErbB3 antibody, p45-sErbB3 antibody, p85-sErbB3 antibody, ERBB3 antibody, C76256 antibody, Erbb-3 antibody, Erbb3r antibody, Her3 antibody, nuc-ErbB3 antibody, erb-b2 receptor tyrosine kinase 3 antibody, v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) antibody, ERBB3 antibody, Erbb3 antibody

背景

ErbB3 is a member of the EGFR/erbB receptor tyrosine kinase family. ErbB3 lacks intrinsic kinase activity due to an amino acid substitution within the ATP binding site. Upon heterodimerization with the other erbB family members, erbB3 becomes phosphorylated at multiple tyrosine residues that represent docking sites for downstream signaling proteins, e.g. PI3K, grb7 and shc. The erbB3 cytoplasmic domain contains 6 docking sites for PI3K. Thus, erbB3 is a potent activator of the PI3K/PKB signaling pathway.Synonyms: ERBB-3, HER-3, Proto-oncogene-like protein c-ErbB-3, Receptor tyrosine-protein kinase erbB-3, Tyrosine kinase-type cell surface receptor HER3, c-erbB-3

分子量

185 kDa

基因ID

2065

UniProt

P21860

途径

RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway