我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

EGFR 抗体 (pTyr1173)

Name: EGFR 抗体 (pTyr1173)
SKU: ABIN126779
Availability: OutOfStock

EGFR 适用: 人, 小鼠, 犬 WB, IF, IHC (p), IP, IHC (fro), EIA 宿主: 小鼠 Monoclonal 9H2 unconjugated

Western Blotting (WB) image for anti-Epidermal Growth Factor Receptor (EGFR) (pTyr1173) antibody (ABIN126779) (EGFR 抗体 (pTyr1173))

1 image

产品编号 ABIN126779

发货至: 中国

Datasheet as PDF

抗原 See all EGFR 抗体

EGFR (Epidermal Growth Factor Receptor (EGFR))
抗原表位
41

39

39

38

35

28

27

26

22

21

19

19

18

17

16

16

15

15

15

14

14

13

13

11

9

8

7

7

7

6

5

5

5

4

4

4

4

4

4

4

3

3

3

3

3

3

2

2

2

2
pTyr1173
适用
805

327

316

26

26

24

19

15

9

3

3

2

1

1

1

1

1

1

1
人, 小鼠, 犬
宿主
635

173

14

11

5

1
小鼠
Compatible Secondaries
克隆类型
583

251

2
单克隆
标记
427

63

32

30

29

29

29

27

27

25

23

15

10

10

10

10

10

5

4

4

4

4

4

1

1

1

1

1

1

1

1
This EGFR antibody is un-conjugated
应用范围
563

262

146

144

136

101

100

95

81

55

37

33

33

18

10

8

6

4

4

3

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

特异性

Clone 9H2 specifically interacts with the 1170 - N A E pY L R V motif corresponding to the major autophosphorylation site of Human EGFR. Antibody AM00042PU-N does not crossreact with the highly homologous pTyr1248 of acticated ErbB2.

产品特性

Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1, Receptor tyrosine-proteinkinase erbB-1, Proto-oncogene c-ErbB-1

纯化方法

Purified from Serum-Free Cell Culture Supernatant by Subsequent Thiophilic Adsorption and Size Exclusion Chromatography

组件

incl. positive Control

免疫原

Phosphopeptide conjugated to KLHEpitope: phospho-Y1197 N A E pY L R V

克隆位点

9H2

亚型

IgG1

Top Product

Discover our top product EGFR Primary Antibody
anti-Epidermal Growth Factor Receptor (EGFR) antibody
EGFR 适用: 人 WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893), (Mutant) antibody
EGFR 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 5G9B5 unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (AA 888-1210) antibody
EGFR 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C4 unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (C-Term) antibody
EGFR 适用: 人, 小鼠, 大鼠, 猴, Pig, Cow, 小鸡, 犬, 马, 兔, 豚鼠, Avian, Cat, 驴, 山羊, Hamster, 绵羊 WB 宿主: 山羊 Polyclonal unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (pSer1026) antibody
EGFR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (pSer695) antibody
EGFR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (pSer1071) antibody
EGFR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (AA 960-1060) antibody
EGFR 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (AA 1021-1210) antibody
EGFR 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (AA 1021-1210) antibody
EGFR 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated

应用备注

Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer. Usecelllysate delivered with this product as positive control: see protocol below! Immunoprecipitation (use at 1-10 μg per 10^6 vanadate-treated A431 cells). Immunocytochemistry (use at 1-10 μg/mL). Immunohistochemistry on Frozen and Paraffin Embedded Material. ELISA (use at 0.05 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

实验流程

Positive Control cell lysate provided: HepG2 Pervanadate Treated-recommended for ImmunoblottingFormulation: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15 min. withpervanadate. Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGEsample buffer, mix and incubate at 90°C for 5 min. Store in aliquots at -20°C. Avoid repeated freezing and thawing. Application: 20 µl of positive cell lysate correspond to ca. 80. 000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysate contains SDS and is not recommended for applications withnative proteins such as immunoprecipitation !

限制

仅限研究用
溶解方式

Restore with 1.0 mL H2O for 15 min at RT

缓冲液

PBS containing 0.09 % Sodium Azide, PEG and Sucrose

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Avoid repeated freeze / thaw cycles!

有效期

12 months
抗原

EGFR (Epidermal Growth Factor Receptor (EGFR))

别名

EGFR / ERBB1 (EGFR 产品)

别名

C-erb antibody, CG10079 antibody, D-EGFR antibody, D-Egf antibody, DEGFR antibody, DER antibody, DER flb antibody, DER/EGFR antibody, DER/faint little ball antibody, DER/top antibody, DER/torpedo antibody, DER1 antibody, DEgfr antibody, Degfr antibody, Der antibody, DmHD-33 antibody, Dmel\\CG10079 antibody, EFG-R antibody, EGF-R antibody, EGFR antibody, EGFr antibody, EGfr antibody, EK2-6 antibody, Egf antibody, Egf-r antibody, EgfR antibody, El antibody, Elp antibody, Elp-1 antibody, Elp-B1 antibody, Elp-B1RB1 antibody, HD-33 antibody, TOP antibody, Torpedo/DER antibody, Torpedo/Egfr antibody, c-erbB antibody, d-egf-r antibody, dEGFR antibody, dEGFR1 antibody, dEgfr antibody, der antibody, egfr antibody, flb antibody, l(2)05351 antibody, l(2)09261 antibody, l(2)57DEFa antibody, l(2)57EFa antibody, l(2)57Ea antibody, mor1 antibody, top antibody, top/DER antibody, top/flb antibody, torpedo/Egfr antibody, torpedo/egfr antibody, EGFR12 antibody, EGFR15 antibody, egfr1 antibody, Erbb2 antibody, ERBB antibody, ERBB1 antibody, HER1 antibody, PIG61 antibody, mENA antibody, ErbB-1 antibody, Errp antibody, 9030024J15Rik antibody, AI552599 antibody, Erbb antibody, Errb1 antibody, Wa5 antibody, wa-2 antibody, wa2 antibody, epidermal growth factor receptor antibody, Epidermal growth factor receptor antibody, epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) antibody, EGFR antibody, Egfr antibody, egfra antibody, egfr1 antibody, LOC5564544 antibody

背景

EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity, phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1

基因ID

1956

UniProt

P00533

途径

NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins