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EGFR 抗体 (pTyr1172)

Name: EGFR 抗体 (pTyr1172)
SKU: ABIN126775
Availability: OutOfStock

This anti-EGFR antibody is a 小鼠单克隆 antibody detecting EGFR in WB. Suitable for 人. This Primary Antibody has been cited in 2+ publications.

Western Blotting (WB) image for anti-Epidermal Growth Factor Receptor (EGFR) (pTyr1172) antibody (ABIN126775) (EGFR 抗体 (pTyr1172))

1 image

(2 references)

产品编号 ABIN126775

发货至: 中国

Quick Overview for EGFR 抗体 (pTyr1172) (ABIN126775)

抗原

See all EGFR 抗体

EGFR (Epidermal Growth Factor Receptor (EGFR))

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This EGFR antibody is un-conjugated

应用范围

Western Blotting (WB)

克隆位点

10G12

Discover our top product EGFR Primary Antibody

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pTyr1172

特异性

This antibody specifically recognizes EGFR phosphorylated at Tyrosine 1172.

产品特性

Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1, Receptor tyrosine-proteinkinase erbB-1, Proto-oncogene c-ErbB-1

纯化方法

Subsequent ultrafiltration and size exclusion

组件

incl. positive Control

免疫原

Phosphopeptide conjugated to hemocyaninN P D pY Q Q D

亚型

IgG
anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893) antibody
EGFR 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 7A6F12 unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (AA 888-1210) antibody
EGFR 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C4 unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (C-Term) antibody
EGFR 适用: 人, 小鼠, 大鼠, Pig, 猴, Cow, 小鸡, 犬, 马, 兔, 豚鼠, Avian, Cat, 驴, 山羊, Hamster, 绵羊 WB 宿主: 山羊 Polyclonal unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (pSer1071) antibody
EGFR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (pSer695) antibody
EGFR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (pSer1026) antibody
EGFR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (AA 1021-1210) antibody
EGFR 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893) antibody
EGFR 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 5E10D3 unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (C-Term) antibody
EGFR 适用: 人, 大鼠 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (AA 888-1210) antibody
EGFR 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

Western blot (0.5 μg/mL for HRPO/ECL detection, recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer). Use cell lysate from EGF-treated HepG2 cells as positive control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

实验流程

Positive control cell lysate provided: HepG2 EGF Treated - recommended for immunoblottingFormulation: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15 min. withEGF. Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGEsample buffer, mix and incubate at 90°C for 5 min. Store in aliquots at -20°C. Avoidrepeated freezing and thawing. Applications: 20 µl of positive cell lysate correspond to ca. 80. 000 cells. Use 20 µl / lane (mini gel) forHRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysate contains SDS and is not recommended for applications withnative proteins such as immunoprecipitation.

限制

仅限研究用
溶解方式

Reconstitute with 1 mL H2O (15 min, RT).

缓冲液

PBS, 0.09 % Na-azide, PEG and Sucrose

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Aliquot and store at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

有效期

12 months
Song, Yu, Sun, Feng, Yu, Khan, Zhu, Huang, Li, Mok, Cheng, Gao, Feng: "Androgen receptor drives hepatocellular carcinogenesis by activating enhancer of zeste homolog 2-mediated Wnt/β-catenin signaling." in: EBioMedicine, Vol. 35, pp. 155-166, (2019) (PubMed).

Yu, Feng, Sun, Zhuo, Li, Zhou, Huang, Jiang, Zhu, Zhang, Le, Zheng, Cheng, Gao: "Bufalin suppresses hepatocarcinogenesis by targeting β-catenin/TCF signaling via cell cycle-related kinase." in: Scientific reports, Vol. 8, Issue 1, pp. 3891, (2018) (PubMed).
抗原

EGFR (Epidermal Growth Factor Receptor (EGFR))

别名

EGFR / ERBB1

背景

EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity, phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1

基因ID

1956

UniProt

P00533

途径

NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins