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EGFR 抗体 (pTyr1110)

Name: EGFR 抗体 (pTyr1110)
SKU: ABIN126772
Availability: OutOfStock

This 小鼠单克隆 antibody specifically detects EGFR in WB 和 EIA. It exhibits reactivity toward 人.

Western Blotting (WB) image for anti-Epidermal Growth Factor Receptor (EGFR) (pTyr1110) antibody (ABIN126772) (EGFR 抗体 (pTyr1110))

1 image

产品编号 ABIN126772

发货至: 中国

Contact our Customer Service for availability and price in your country. Contact Info

Our Local Distributor

中国
北京 101111

No. 88 KeChuang 6th Street

Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for EGFR 抗体 (pTyr1110) (ABIN126772)

抗原

See all EGFR 抗体

EGFR (Epidermal Growth Factor Receptor (EGFR))

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This EGFR antibody is un-conjugated

应用范围

Western Blotting (WB), Enzyme Immunoassay (EIA)

克隆位点

8B8

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pTyr1110

特异性

Thisa antibody specifically recognizes EGFR phosphorylated at Tyrosine 1110. It does not interact with the non-phosphorylated EGFR nor with unrelated Tyrosine-phosphorylated proteins.

产品特性

Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1, Receptor tyrosine-proteinkinase erbB-1, Proto-oncogene c-ErbB-1

纯化方法

Subsequent ultrafiltration and size exclusion chromatography

免疫原

Synthetic phosphopeptide conjugated to hemocyaninEpitope: phosphotyrosine 1110, N P V pY H N Q

亚型

IgG2b
anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893) antibody
EGFR 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 7A6F12 unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (AA 888-1210) antibody
EGFR 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C4 unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (C-Term) antibody
EGFR 适用: 人, 小鼠, 大鼠, 猴, Pig, Cow, 小鸡, 犬, 马, 兔, 豚鼠, Avian, Cat, 驴, 山羊, Hamster, 绵羊 WB, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (pSer1071) antibody
EGFR 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (pSer1026) antibody
EGFR 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (pSer695) antibody
EGFR 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (AA 693-893) antibody
EGFR 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 5E10D3 unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) antibody
KD Validated EGFR 适用: 人 WB, FACS, IF (p) 宿主: 兔 Monoclonal 24GB4395 unconjugated Recombinant Antibody

anti-Epidermal Growth Factor Receptor (EGFR) (C-Term) antibody
EGFR 适用: 人, 大鼠 WB, IHC, ELISA, IP 宿主: 兔 Polyclonal unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (AA 888-1210) antibody
EGFR 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

Western blot (1 μg/mL for HRPO/ECL detection, recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer). ELISA (0.1 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

实验流程

Positive controlFormat: Lyophilized cell lysate from A431 cells. Serum starved cells were treated for 15 minwith pervanadate. Reconstitute by addition of 200 µl H20. After complete solubilization add 200 µl 2xSDS-PAGE sample buffer, mix and incubate at 90° C for 5 min. Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl / lane (minigel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation.

限制

仅限研究用
溶解方式

Reconstitute in 1 mL distilled water for 15 min at RT

缓冲液

PBS, 0.09 % Na-azide, PEG and Sucrose

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C/-20 °C

储存方法

Store lyophilised product upon arrival at -20 °C. Following reconstitution aliquot and store at 2 - 8 °C for up to three months or freeze inliquid nitrogen at -80 °C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate, we recommend centrifugation before use.
Shelf life: One year from despatch.

有效期

12 months
抗原

EGFR (Epidermal Growth Factor Receptor (EGFR))

别名

EGFR / ERBB1

背景

EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity, phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1

基因ID

1956

UniProt

P00533

途径

NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins