EGFR
适用: 人
WB, IHC, IP
宿主: 兔
Polyclonal
unconjugated
应用备注
ELISA (protein ELISA: 0.1 μg/mL). Western blot (1 μg/mL for HRPO/ECL detection, recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer). Immunoprecipitation (1 - 10 g per 10^6 pervanadate-treated A431 cells). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
实验流程
Positive controlFormat: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for15 min with pervanadate. Reconstitute by addition of 200 µl H20. After complete solubilization add 200 µl 2xSDS-PAGE sample buffer, mix and incubate at 90 °C for 5 min. Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80. 000 cells. Use 20 µl / lane (minigel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation.
限制
仅限研究用
溶解方式
Restore with 1 mL H2O, 15 min, RT.
缓冲液
PBS, 0.09 % Na-azide, PEG and Sucrose
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-20 °C
储存方法
Store lyophilised product upon arrival at -20 °C. Following reconstitution aliquot and store at 2 - 8 °C for up to three months or freeze inliquid nitrogen at -80 °C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate, we recommend centrifugation before use. Shelf life: One year from despatch.
EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity, phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1