EGFR
适用: 人
WB, IHC, IP
宿主: 兔
Polyclonal
unconjugated
应用备注
Western Blot: 0.5 μg/mL for HRPO/ECL detection. Positive control: Cell lysate from untreated HepG2 cells (included - please see Protocols). Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
实验流程
Positive Control Cell Lysate: HepG2 UntreatedFormat: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Applications: The positive control cell lysate is recommended for immunoblotapplications. 20 µl of positive control cell lysate correspond to ca. 80,000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation.
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
PBS/0.09 % Na-Azide/PEG and Sucrose
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C
储存方法
Aliquote and store the antibody (in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y845 by src family kinases. Phosphorylation of Y1045 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1047 by CamKinase II leads to attenuation of kinase activity, phosphorylation of T654 (by PKC) and T669 (by MAPK, p38) interferes with receptor endocytosis/recycling.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1