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EGFR 抗体 (pThr678)

Name: EGFR 抗体 (pThr678)
SKU: ABIN126757
Availability: OutOfStock

EGFR 适用: 人, 小鼠, 大鼠, 犬 WB, IP, EIA 宿主: 小鼠 Monoclonal 3F2 unconjugated

Western Blotting (WB) image for anti-Epidermal Growth Factor Receptor (EGFR) (pThr678) antibody (ABIN126757) (EGFR 抗体 (pThr678))

1 image

产品编号 ABIN126757

发货至: 中国

Datasheet as PDF

抗原 See all EGFR 抗体

EGFR (Epidermal Growth Factor Receptor (EGFR))
抗原表位
39

39

38

38

28

27

27

23

21

21

19

18

17

16

16

16

15

15

15

14

14

13

13

11

9

8

7

7

7

6

5

5

5

4

4

4

3

3

3

3

3

3

3

3

3

2

2

2

2

2
pThr678
适用
772

319

309

26

26

24

19

14

9

3

2

2

1

1

1

1

1

1
人, 小鼠, 大鼠, 犬
宿主
610

166

14

11

4

1
小鼠
Compatible Secondaries
克隆类型
575

226

2
单克隆
标记
399

63

31

29

29

29

29

27

27

25

22

14

10

10

10

10

10

5

4

4

4

4

4

1

1

1

1

1

1

1
This EGFR antibody is un-conjugated
应用范围
539

254

145

134

122

101

91

85

81

42

37

33

33

18

10

8

6

4

4

3

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

特异性

This antibody specifically recognizes EGFR phosphorylated at Threonine 678.

产品特性

Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1, Receptor tyrosine-proteinkinase erbB-1, Proto-oncogene c-ErbB-1

纯化方法

Subsequent thiophilic adsorption and size exclusion chromatography

组件

incl. positive Control

免疫原

Phosphopeptide conjugated to KLH. Remarks: Epitope: Phosphothreonine 678, R K R pT L R R

克隆位点

3F2

亚型

IgG1

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anti-Epidermal Growth Factor Receptor (EGFR) (C-Term) antibody
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anti-Epidermal Growth Factor Receptor (EGFR) (pSer1071) antibody
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anti-Epidermal Growth Factor Receptor (EGFR) (AA 960-1060) antibody
EGFR 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (AA 1021-1210) antibody
EGFR 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Epidermal Growth Factor Receptor (EGFR) (AA 1021-1210) antibody
EGFR 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated

应用备注

ELISA (0.05 μg/mL). Western blot. Immunoprecipitation (1 - 10 μg per 10^6 vanadate treated A431 cells). Luminex.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

实验流程

Positive controlFormat: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15min with pervanadate plus PMA. Reconstitution: Reconstitute by addition of 200 µl H2O. After complete solubilization add200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90 °C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl positive control cell lysate correspond to approx. 20. 000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplicatioins with native proteins such as Immunoprecipitation. Storage: Aliquot and store frozen. Avoid repeated freeze/thaw cycles.

限制

仅限研究用
浓度

0.1 mg/mL

缓冲液

PBS, 0.09 % Na-Azide, PEG and Sucrose, 50 % Glycerol

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

有效期

12 months
抗原

EGFR (Epidermal Growth Factor Receptor (EGFR))

别名

EGFR / ERBB1 (EGFR 产品)

别名

C-erb antibody, CG10079 antibody, D-EGFR antibody, D-Egf antibody, DEGFR antibody, DER antibody, DER flb antibody, DER/EGFR antibody, DER/faint little ball antibody, DER/top antibody, DER/torpedo antibody, DER1 antibody, DEgfr antibody, Degfr antibody, Der antibody, DmHD-33 antibody, Dmel\\CG10079 antibody, EFG-R antibody, EGF-R antibody, EGFR antibody, EGFr antibody, EGfr antibody, EK2-6 antibody, Egf antibody, Egf-r antibody, EgfR antibody, El antibody, Elp antibody, Elp-1 antibody, Elp-B1 antibody, Elp-B1RB1 antibody, HD-33 antibody, TOP antibody, Torpedo/DER antibody, Torpedo/Egfr antibody, c-erbB antibody, d-egf-r antibody, dEGFR antibody, dEGFR1 antibody, dEgfr antibody, der antibody, egfr antibody, flb antibody, l(2)05351 antibody, l(2)09261 antibody, l(2)57DEFa antibody, l(2)57EFa antibody, l(2)57Ea antibody, mor1 antibody, top antibody, top/DER antibody, top/flb antibody, torpedo/Egfr antibody, torpedo/egfr antibody, EGFR12 antibody, EGFR15 antibody, egfr1 antibody, Erbb2 antibody, ERBB antibody, ERBB1 antibody, HER1 antibody, PIG61 antibody, mENA antibody, ErbB-1 antibody, Errp antibody, 9030024J15Rik antibody, AI552599 antibody, Erbb antibody, Errb1 antibody, Wa5 antibody, wa-2 antibody, wa2 antibody, epidermal growth factor receptor antibody, Epidermal growth factor receptor antibody, epidermal growth factor receptor a (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) antibody, EGFR antibody, Egfr antibody, egfra antibody, egfr1 antibody, LOC5564544 antibody

背景

EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity, phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1

基因ID

1956

UniProt

P00533

途径

NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins