CK1 epsilon 抗体 (AA 355-380)
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北京 101111
Quick Overview for CK1 epsilon 抗体 (AA 355-380) (ABIN126752)
抗原
See all CK1 epsilon (CSNK1E) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 355-380
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特异性
- Mab CK1e-4D7 specifically recognizes the epsilon isoform of CK1.
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产品特性
- Synonyms: CKI-epsilon, CKIe, CSNK1E
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纯化方法
- Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
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组件
- incl. positive Control
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免疫原
- Peptide (aa 355 - 380 of CK1e) conjugated to hemocyanin
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亚型
- IgG1
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anti-Casein Kinase 1, epsilon (CSNK1E) (C-Term) antibody
Verified CSNK1E 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Casein Kinase 1, epsilon (CSNK1E) antibodyKD Validated CSNK1E 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 25GB490 unconjugated
anti-Casein Kinase 1, epsilon (CSNK1E) antibodyCSNK1E 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Casein Kinase 1, epsilon (CSNK1E) (Internal Region) antibodyCSNK1E 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Casein Kinase 1, epsilon (CSNK1E) (AA 1-416), (Isoform epsilon) antibodyCSNK1E 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Casein Kinase 1, epsilon (CSNK1E) (AA 17-231), (Isoform epsilon) antibodyCSNK1E 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Casein Kinase 1, epsilon (CSNK1E) (AA 2-100) antibodyCSNK1E 适用: 人 ELISA, IF, PLA 宿主: 小鼠 Monoclonal 2E1 unconjugated
anti-Casein Kinase 1, epsilon (CSNK1E) (AA 331-360), (C-Term) antibodyCSNK1E 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB0845 unconjugated
anti-Casein Kinase 1, epsilon (CSNK1E) (AA 276-325) antibodyCSNK1E 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Casein Kinase 1, epsilon (CSNK1E) (Center) antibodyCSNK1E 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA (0.1 μg/mL). Western blot (detects a band at 45 kD, Use at 0.5 μg/mL for HRPO/ECL detection,Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- For positive control cell lysate from serum starved A431 cells.
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限制
- 仅限研究用
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溶解方式
- Reconstitute with 1 mL H2O (15 min, RT)
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缓冲液
- 2 x PBS / 0.09 % Na-azide / PEG and Sucrose
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
- For long term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution aliquoteand freeze in liquid nitrogen, reconstituted antibody can be stored frozen at -80 °C up to 1year. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months. Avoidrepeated freeze/thaw cycles.
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有效期
- 12 months
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- CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))
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别名
- Casein Kinase I epsilon
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背景
- Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as subtrates. It can also phosphorylate a large number of proteins and participates in Wnt signaling.Synonyms: CKI-epsilon, CKIe, CSNK1E
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基因ID
- 1454
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UniProt
- P49674
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途径
- Hedgehog Signaling, M Phase
抗原
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