GFP 抗体
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北京 101111
Quick Overview for GFP 抗体 (ABIN121945)
抗原
See all GFP 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- The antibody reacts specifically with GFP, EGFP, EYFP fusion proteins in all species.
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纯化方法
- Affinity Chromatography on Protein A
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免疫原
- EGFP, a native full-length protein
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anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea aequorea WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6C11C11 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IP, IC, ChIP-seq 宿主: 大鼠 Monoclonal 1A5 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IF, IHC (fro), IHC (p), IEM 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IHC, IP, ICC, FACS 宿主: 小鼠 Monoclonal 4B10B2 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IHC, IP, FM, FACS, DB 宿主: 小鼠 Monoclonal 9F9-F9 unconjugated
anti-Green Fluorescent Protein (GFP) (AA 1-100) antibodyGFP 适用: Aequorea victoria WB, IF 宿主: 小鼠 Monoclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibody (DyLight 550)GFP 适用: Aequorea victoria WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 550
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunoprecipitation (10-20 μg/sample). Immunocytochemistry (1-3 μg/mL). Western Blotting (0.5-1.5 μg/mL). Positive control: transfected cells. Negative control: non-transfected cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, 15 mM Sodium Azide, 0.2 % (w/v) high-grade BSA (protease free)
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. -
有效期
- 12 months
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: "Super-resolution imaging of ESCRT-proteins at HIV-1 assembly sites." in: PLoS pathogens, Vol. 11, Issue 2, pp. e1004677, (2016) (PubMed).
: "Distinct functionality of dishevelled isoforms on Ca2+/calmodulin-dependent protein kinase 2 (CamKII) in Xenopus gastrulation." in: Molecular biology of the cell, Vol. 26, Issue 5, pp. 966-77, (2015) (PubMed).
: "CCHCR1 interacts with EDC4, suggesting its localization in P-bodies." in: Experimental cell research, Vol. 327, Issue 1, pp. 12-23, (2014) (PubMed).
: "Expression of endogenous nuclear bradykinin B2 receptors mediating signaling in immediate early gene activation." in: Journal of cellular physiology, Vol. 216, Issue 1, pp. 234-44, (2008) (PubMed).
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: "Super-resolution imaging of ESCRT-proteins at HIV-1 assembly sites." in: PLoS pathogens, Vol. 11, Issue 2, pp. e1004677, (2016) (PubMed).
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- GFP (Green Fluorescent Protein (GFP))
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别名
- GFP
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背景
- Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (509 nm) when excited (excitation by Blue or UV light, absorption peak at 395 nm). GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. To increase a fluorescence intensity of GFP, chromophore mutations have been created. The Enhanced GFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. Yellow Fluorescent Protein, Cyan Fluorescent Protein) with warying spectral properties. Antibodies raised against full-length GFP variants should also detect other variants of the protein.Synonyms: GFP-Tag, Green fluorescent protein
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UniProt
- P42212
抗原
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