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GFP 抗体

Name: GFP 抗体
SKU: ABIN121945
Availability: OutOfStock

This anti-GFP antibody is a 兔多克隆 antibody detecting GFP in WB, IP 和 IF. Suitable for Aequorea victoria. This Primary Antibody has been cited in 4+ publications.

(A) Confocal microscopy images of COS-7 cells transfected with expression constructs encoding membrane-tethered EGFP (membrane-EGFP (GFP 抗体)

Immunoprecipitation of GFP-NLS from HEK293 cells using anti-GFP antibody. (GFP 抗体)

Confocal microscopy images of COS-7 cells transfected with expression constructs encoding membrane-tethered EGFP (membrane-EGFP, top) or nuclear Polycomb 2-EYFP fusion protein (Pc2-EYFP, bottom). (GFP 抗体)

3 images

(4 references)

产品编号 ABIN121945

发货至: 中国

Quick Overview for GFP 抗体 (ABIN121945)

抗原

See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))

适用

Aequorea victoria

宿主

兔

克隆类型

多克隆

标记

This GFP antibody is un-conjugated

应用范围

Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Discover our top product GFP Primary Antibody

特异性

The antibody reacts specifically with GFP, EGFP, EYFP fusion proteins in all species.

纯化方法

Affinity Chromatography on Protein A

免疫原

EGFP, a native full-length protein
anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 山羊 Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea aequorea WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6C11C11 unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, IP, IC, ChIP-seq 宿主: 大鼠 Monoclonal 1A5 unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, IF, IHC (fro), IHC (p), IEM 宿主: 山羊 Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, IHC, IP, ICC, FACS 宿主: 小鼠 Monoclonal 4B10B2 unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, IHC, IP, FM, FACS, DB 宿主: 小鼠 Monoclonal 9F9-F9 unconjugated

anti-Green Fluorescent Protein (GFP) antibody (DyLight 550)
GFP 适用: Aequorea victoria WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 550

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, IHC, IP, FM, FACS 宿主: 兔 Polyclonal unconjugated

应用备注

Immunoprecipitation (10-20 μg/sample). Immunocytochemistry (1-3 μg/mL). Western Blotting (0.5-1.5 μg/mL). Positive control: transfected cells. Negative control: non-transfected cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

1.0 mg/mL

缓冲液

PBS, 15 mM Sodium Azide, 0.2 % (w/v) high-grade BSA (protease free)

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C/-20 °C

储存方法

Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

有效期

12 months
Prescher, Baumgärtel, Ivanchenko, Torrano, Bräuchle, Müller, Lamb: "Super-resolution imaging of ESCRT-proteins at HIV-1 assembly sites." in: PLoS pathogens, Vol. 11, Issue 2, pp. e1004677, (2016) (PubMed).

Gentzel, Schille, Rauschenberger, Schambony: "Distinct functionality of dishevelled isoforms on Ca2+/calmodulin-dependent protein kinase 2 (CamKII) in Xenopus gastrulation." in: Molecular biology of the cell, Vol. 26, Issue 5, pp. 966-77, (2015) (PubMed).

Ling, Wong, Li, Chan, Boukamp, Liu: "CCHCR1 interacts with EDC4, suggesting its localization in P-bodies." in: Experimental cell research, Vol. 327, Issue 1, pp. 12-23, (2014) (PubMed).

Savard, Barbaz, Bélanger, Müller-Esterl, Bkaily, Dorléans-Juste, Coté, Bovenzi, Gobeil: "Expression of endogenous nuclear bradykinin B2 receptors mediating signaling in immediate early gene activation." in: Journal of cellular physiology, Vol. 216, Issue 1, pp. 234-44, (2008) (PubMed).
抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP

背景

Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (509 nm) when excited (excitation by Blue or UV light, absorption peak at 395 nm). GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. To increase a fluorescence intensity of GFP, chromophore mutations have been created. The Enhanced GFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. Yellow Fluorescent Protein, Cyan Fluorescent Protein) with warying spectral properties. Antibodies raised against full-length GFP variants should also detect other variants of the protein.Synonyms: GFP-Tag, Green fluorescent protein

UniProt

P42212