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SYK 抗体 (AA 5-360)

Name: SYK 抗体 (AA 5-360)
SKU: ABIN120732
Availability: OutOfStock

This anti-SYK antibody is a 小鼠单克隆 antibody detecting SYK in WB, IF, IP 和 IHC (p). Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 3+ publications.

(3 references)

产品编号 ABIN120732

发货至: 中国

Quick Overview for SYK 抗体 (AA 5-360) (ABIN120732)

抗原

See all SYK 抗体

SYK (Spleen tyrosine Kinase (SYK))

适用

人, 小鼠, 大鼠

宿主

小鼠

克隆类型

单克隆

标记

This SYK antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

SYK-01

Discover our top product SYK Primary Antibody

抗原表位
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AA 5-360

特异性

The monoclonal SYK-01 antibody reacts with Protein Tyrosine Kinase p72Syk (Syk, Syk family tyrosine-specific phospho-transferase), which is required for the transduction of signals through the B cell antigen receptor (BCR) and the high affinity IgE receptor (FceRI).

纯化方法

Affinity Chromatography on Protein A

免疫原

Recombinant fragment (aa 5-360) of human Syk

亚型

IgG1
anti-Spleen tyrosine Kinase (SYK) (AA 5-360) antibody
SYK 适用: 人, 小鼠, 大鼠 WB, IP, IHC (p), ICC 宿主: 小鼠 Monoclonal SYK-01 unconjugated

anti-Spleen tyrosine Kinase (SYK) (pTyr525) antibody
SYK 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Spleen tyrosine Kinase (SYK) (pTyr348) antibody
SYK 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Spleen tyrosine Kinase (SYK) (pTyr323) antibody
SYK 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Spleen tyrosine Kinase (SYK) (Internal Region) antibody
SYK 适用: 人, 小鼠, 大鼠 WB, IF, IP, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Spleen tyrosine Kinase (SYK) (AA 217-356) antibody
SYK 适用: 人 ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 2G11E6 unconjugated

anti-Spleen tyrosine Kinase (SYK) (AA 217-356) antibody
SYK 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 3D1G10 unconjugated

anti-Spleen tyrosine Kinase (SYK) (AA 387-417), (C-Term) antibody
SYK 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB03097 unconjugated

anti-Spleen tyrosine Kinase (SYK) (pTyr525), (pTyr526) antibody
SYK 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Spleen tyrosine Kinase (SYK) (pTyr323) antibody
SYK 适用: 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

Immunocytochemistry. Immunoprecipitation. Western blot: Recommended Dilution: 1-2 mg/mL, 60 min, Positive Control: RBL rat basophilic leukemia cell line, A-431 human epidermoid carcinoma cell line, RAMOS lymphoma cell line, U-937 human histiocytic lymphoma cell line, JURKAT human peripheral blood T cell leukemia cell line. Negative Control: HeLa human cervix carcinoma cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate (1:1) with non- reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (12 % separating gel).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

1.0 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH ~7.4, 15 mM Sodium Azide

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C/-20 °C

储存方法

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

有效期

12 months
Heneberg, Lebduska, Dráberová, Korb, Dráber: "Topography of plasma membrane microdomains and its consequences for mast cell signaling." in: European journal of immunology, Vol. 36, Issue 10, pp. 2795-806, (2006) (PubMed).

Hálová, Dráberová, Dráber: "A novel lipid raft-associated glycoprotein, TEC-21, activates rat basophilic leukemia cells independently of the type 1 Fc epsilon receptor." in: International immunology, Vol. 14, Issue 2, pp. 213-23, (2002) (PubMed).

Dráberová, Dráber, Havlícek, Viklický: "A common antigenic determinant of vimentin and desmin defined by monoclonal antibody." in: Folia biologica, Vol. 32, Issue 5, pp. 295-303, (1989) (PubMed).
抗原

SYK (Spleen tyrosine Kinase (SYK))

别名

SYK

背景

Syk is a cytoplasmic protein tyrosine kinase that translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FceRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.Synonyms: Spleen tyrosine kinase, Tyrosine-protein kinase SYK

基因ID

6850

UniProt

P43405

途径

Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling, BCR Signaling