CD11b 抗体
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- 抗原 See all CD11b (ITGAM) 抗体
- CD11b (ITGAM) (Integrin alpha M (ITGAM))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD11b antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP)
- 特异性
- The antibody recognizes CD11b antigen (Mac-1), a 165 kDa type I transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.
- 交叉反应 （详细）
- Species reactivity (tested):Human
- 纯化方法
- Affinity Chromatography on Protein A
- 免疫原
- Human granulocytes
- 克隆位点
- MEM-174
- 亚型
- IgG2a
- Top Product
- Discover our top product ITGAM Primary Antibody
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anti-Integrin alpha M (ITGAM) (AA 520-570) antibody
ITGAM 适用: 人, 小鼠, 大鼠 FACS 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha M (ITGAM) (AA 145-340) antibodyITGAM 适用: 人 IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha M (ITGAM) (AA 77-180) antibodyITGAM 适用: 人, 小鼠, 大鼠 FACS, WB, IHC (fro), ELISA, IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha M (ITGAM) (AA 253-282), (N-Term) antibodyITGAM 适用: 人 FACS, WB, IHC (p) 宿主: 兔 Polyclonal RB17991 unconjugated
anti-Integrin alpha M (ITGAM) (AA 31-160) antibodyITGAM 适用: 大鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha M (ITGAM) (AA 941-1074) antibodyITGAM 适用: 人 IHC, WB, StM 宿主: 小鼠 Monoclonal ITGAM-3338 unconjugated
anti-Integrin alpha M (ITGAM) (AA 941-1074) antibodyITGAM 适用: 人 IHC, WB, StM 宿主: 小鼠 Monoclonal ITGAM-3340 unconjugated
anti-Integrin alpha M (ITGAM) antibody (FITC)ITGAM 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-174 FITC
anti-Integrin alpha M (ITGAM) antibody (Biotin)ITGAM 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-174 Biotin
anti-Integrin alpha M (ITGAM) antibody (PE)ITGAM 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-174 PE
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- 应用备注
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Flow Cytometry (Positive control: Peripheral Blood Lymphocytes). Immunoprecipitation: Positive control: Granulocytes CD11b/CD18 transfectants Application note: Immunoprecipitation with the antibody ABIN120554 was successfully performed using immuno-affinity sorbents (minicolumns). The final immunosorbent should contain 1-5 mg of antibody/1 mLof sorbent gel (e.g. agarose beads). It is recommended to stimulate positive control material by LPS 1-2 days before to achieve better expression of target antigen).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH ~7.4 with 15 mM Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. - 有效期
- 12 months
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- 抗原
- CD11b (ITGAM) (Integrin alpha M (ITGAM))
- 别名
- CD11b (ITGAM 产品)
- 背景
- CD11b (integrin aM subunit) is a 165 kDa type I transmembrane glycoprotein that non-covalently associates with integrin b2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor
- 基因ID
- 3684
- UniProt
- P11215
- 途径
- Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
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