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His Tag 抗体 (HRP)

WB, EIA 宿主: 小鼠 Monoclonal AD1-1-10 HRP
产品编号 ABIN119352
发货至: 中国
  • 抗原 See all His Tag 抗体
    His Tag
    适用
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    宿主
    • 92
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    小鼠
    克隆类型
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    • 10
    单克隆
    标记
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    This His Tag antibody is conjugated to HRP
    应用范围
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    Western Blotting (WB), Enzyme Immunoassay (EIA)
    序列
    HHHHHH
    纯化方法
    Affinity Chromatography on Protein G
    免疫原
    PAX6 transcription factor linked to histidine tag. Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
    克隆位点
    AD1-1-10
    亚型
    IgG1
    Top Product
    Discover our top product His Tag Primary Antibody
  • 应用备注
    ELISA: 1/20 - 1/1000. Western Blot: 1/1000 - 1/5000, In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    PBS, pH 7.4 containing 0.01 % Thiomersal as preservative and a HRP stabilizer
    储存条件
    4 °C
    储存方法
    Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
  • 抗原
    His Tag
    别名
    6xHistidine Epitope Tag (His Tag 产品)
    物质类
    Tag
    背景
    6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems.
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