CD59 抗体
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- 抗原 See all CD59 抗体
- CD59
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD59 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Reacts with a PI-linked glycoprotein, Mw 18-20 kDa found on all types of leucocytes including platelets. The antigen is expressed on many haemapoietic and non-haemapoietic cells. MEM-43 has been shown to stimulate NK activity. CD59 is expressed by erythrocytes and therefore staining in whole blood systems requires a high concentration of antibody. The epitope recognised by MEM-43 is lost after reduction, therefore, non-reducing conditions are required for western blotting techniques (2, 5).
- 纯化方法
- Affinity Chromatography on Protein A.
- 克隆位点
- MEM-43
- 亚型
- IgG2a
- Top Product
- Discover our top product CD59 Primary Antibody
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anti-CD59 (CD59) (AA 31-111) antibody
CD59 适用: 人 FACS, WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 8D2B8 unconjugated
anti-CD59 (CD59) (AA 74-110) antibodyCD59 适用: 人 FACS, WB, IF, IHC (p) 宿主: 兔 Polyclonal RB56691 unconjugated
anti-CD59 (CD59) antibodyCD59 适用: 人 FACS, IP, IHC (p) 宿主: 小鼠 Monoclonal MEM-43 unconjugated
anti-CD59 (CD59) antibodyCD59 适用: 人, 小鼠 FACS, WB, IP, IHC (p) 宿主: 小鼠 Monoclonal MEM-43-5 unconjugated
anti-CD59 (CD59) (AA 31-111) antibodyCD59 适用: 人 FACS, WB, IHC, ELISA, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
anti-CD59 (CD59) (AA 23-105) antibodyCD59 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD59 (CD59) (AA 1-128) antibodyCD59 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3G6 unconjugated
anti-CD59 (CD59) (AA 52-100) antibodyCD59 适用: 人 FACS, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD59 (CD59) (AA 26-102) antibodyCD59 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-CD59 (CD59) antibodyCD59 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA. Immunoprecipitation. Western blot (non-reducing conditions). Flow Cytometry (Use 10 μL of 1/5-1/10 diluted antibody to label 106 cells in 100 μL). Immunohistochemistry on Frozen and Paraffin Sections: Requires antigen retrieval using heat treatment methods prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. Positive control: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS with 0.09 % Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- CD59
- 别名
- CD59 (CD59 产品)
- 别名
- 16.3A5 antibody, 1F5 antibody, EJ16 antibody, EJ30 antibody, EL32 antibody, G344 antibody, HRF-20 antibody, HRF20 antibody, MAC-IP antibody, MACIF antibody, MEM43 antibody, MIC11 antibody, MIN1 antibody, MIN2 antibody, MIN3 antibody, MIRL antibody, MSK21 antibody, p18-20 antibody, Cd59a antibody, Cd59b antibody, MACIP antibody, CD59 molecule (CD59 blood group) antibody, CD59 molecule antibody, CD59 antibody, Cd59 antibody
- 背景
- CD59, an LY6 like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells. It is a potent inhibitor of the complement membrane attack complex action. It acts by binding to the C8 and/or C9 complements of the membrane attack complex, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is also involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.Synonyms: 20 kDa homologous restriction factor, HRF-20, HRF20, MAC-IP, MAC-inhibitory protein, MACIF, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin
- 基因ID
- 966
- NCBI登录号
- NP_000602
- UniProt
- P13987
- 途径
- Complement System
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