RPA2 抗体 (AA 115-129)
Quick Overview for RPA2 抗体 (AA 115-129) (ABIN118008)
抗原
See all RPA2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 115-129
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序列
- Peptide sequence: S-S-G-K-G-Y-G-S-Q-V-A-Q-Q-F-E
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特异性
- This affinity purified antibody is directed against yeast RFA2. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was purified against the phosphorylated form of the immunizing peptide and then cross adsorbed against the non-phosphorylated form of the peptide. This antibody consists of antibodies eluted from the non-phospho peptide column.
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纯化方法
- Immunoaffinity chromatography.
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免疫原
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 115-129 (of 273) of yeast DNA Replication Factor A (RFA2). The RFA complex consists of a heterotrimer of RFA1, RFA2 and RFA3, 69, 36 and 13 kda chains respectively. Yeast RFA2 is phosphorylated at the G1/S transition and is dephosphorylated at mitosis.
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anti-Replication Protein A2, 32kDa (RPA2) (AA 1-270) antibody
RPA2 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Replication Protein A2, 32kDa (RPA2) antibodyRPA2 适用: 人, 犬, 猴 WB, IHC, IF, IHC (p), IP, FACS 宿主: 小鼠 Monoclonal 7C12 unconjugated
anti-Replication Protein A2, 32kDa (RPA2) antibodyRPA2 适用: 人, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p), IP, FACS 宿主: 小鼠 Monoclonal 1H10 unconjugated
anti-Replication Protein A2, 32kDa (RPA2) antibodyRPA2 适用: 人, 猴 WB, IHC, IF, IHC (p), IP, FACS 宿主: 小鼠 Monoclonal 9A1 unconjugated
anti-Replication Protein A2, 32kDa (RPA2) (AA 1-267) antibodyRPA2 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Replication Protein A2, 32kDa (RPA2) antibodyRPA2 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Replication Protein A2, 32kDa (RPA2) (N-Term) antibodyRPA2 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Replication Protein A2, 32kDa (RPA2) (pThr21) antibodyRPA2 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Replication Protein A2, 32kDa (RPA2) (pThr21) antibodyRPA2 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Replication Protein A2, 32kDa (RPA2) (AA 101-200) antibodyRPA2 适用: 小鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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This affinity-purified antibody was tested for use in western blotting against RFA2containing cell lysates and by ELISA against the immunizing peptide. Reactivity in otherimmunoassays is unknown. This product was assayed by western blotting against RFA2containing cell lysates. A 1: 100 to 1: 500 dilution is recommended for this assay. Thisproduct was also assayed by ELISA against 0.1 μg of the phosphorylated andnonphosphorylated form of the immunizing peptide. A 1: 1,000 to 1: 3,000 dilution of theantibody is recommended against both forms of the peptide for this assay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.12 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 2-8 °C.
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- RPA2 (Replication Protein A2, 32kDa (RPA2))
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别名
- RPA2
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背景
- Synonyms: REPA2, RF-A protein 2, RFA2, RP-A p32, RP-A p34, RPA32, RPA34, Replication factor A protein 2, Replication protein A, Replication protein A 32 kDa subunit, Replication protein A 34 kDa subunit
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基因ID
- 6118
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NCBI登录号
- NP_002937
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UniProt
- P15927
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途径
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
抗原
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