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- 抗原 See all PPARG 抗体
- PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))
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抗原表位
- N-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PPARG antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 序列
- Peptide sequence: M-G-E-T-L-G-D-S-P-I-D-P-E-S-D-S
- 特异性
- This antibody is directed against Human PPAR gamma isoform 2 protein. A BLAST analysis was used to suggest reactivity with this protein from Human and Macaque based on 100 % homology for the immunogen sequence. Cross reactivity with PPARg2 protein from Dog and Swine is likely due to 94 % homology (15/16 identities) with the protein from these sources. Cross reactivity does occur with PPARg2 from Mouse. Mouse PPARg2 shows 87 % homology to the immunogen. Cross reactivity with PPARg2 homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.
- 纯化方法
- Immunoaffinity Chromatography using the immunizing peptide after immobilization to a solid phase.
- 免疫原
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of Human PPAR gamma 2. PPAR gamma 2 is a 55 kDa protein.
- Top Product
- Discover our top product PPARG Primary Antibody
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anti-Peroxisome Proliferator-Activated Receptor gamma (PPARG) (AA 50-150) antibody
PPARG 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Peroxisome Proliferator-Activated Receptor gamma (PPARG) (AA 101-200) antibodyPPARG 适用: 人, 小鼠, 大鼠, Cow, 绵羊 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Peroxisome Proliferator-Activated Receptor gamma (PPARG) (AA 315-420) antibodyPPARG 适用: 人, 小鼠, 大鼠, 山羊 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Peroxisome Proliferator-Activated Receptor gamma (PPARG) (pSer112) antibodyPPARG 适用: 人, 小鼠, 大鼠, 犬 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Peroxisome Proliferator-Activated Receptor gamma (PPARG) (AA 149-273) antibodyPPARG 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Peroxisome Proliferator-Activated Receptor gamma (PPARG) (pSer112) antibodyPPARG 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Peroxisome Proliferator-Activated Receptor gamma (PPARG) (pSer273) antibodyPPARG 适用: 人, 小鼠, 大鼠, 绵羊, 豚鼠 WB, ELISA, ICC, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Peroxisome Proliferator-Activated Receptor gamma (PPARG) (AA 70-170) antibodyPPARG 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Peroxisome Proliferator-Activated Receptor gamma (PPARG) (pSer112) antibodyPPARG 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Peroxisome Proliferator-Activated Receptor gamma (PPARG) antibodyPPARG 适用: 小鼠, 大鼠 IHC 宿主: 小鼠 Monoclonal 6D7B9 unconjugated
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- 应用备注
- This affinity purified antibody has been tested for use in ELISA (1: 4,000-1: 40,000) and byWestern blot (1: 1,000-1: 3,000). Specific conditions for reactivity should be optimized bythe end user. Expect a single band approximately 55 kDa in size corresponding to PPARγ2
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store undiluted at 2-8 °C for one month or (in small aliquots) at -20 °C for longer.
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- 抗原
- PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))
- 别名
- PPAR-gamma (PPARG 产品)
- 别名
- PPAR gamma antibody, PPARg2 antibody, PPARG antibody, Nr1c3 antibody, PPAR-gamma antibody, PPAR-gamma2 antibody, PPARgamma antibody, PPARgamma2 antibody, NR1C3 antibody, CIMT1 antibody, GLM1 antibody, PPARG1 antibody, PPARG2 antibody, xPPAR-gamma antibody, PPAR-GAMMA antibody, PPARGAMMA antibody, peroxisome proliferator-activated receptor gamma antibody, peroxisome proliferator activated receptor gamma antibody, peroxisome proliferator activated receptor gamma L homeolog antibody, PPARG antibody, Pparg antibody, pparg.L antibody
- 背景
- Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. The target genes of PPAR-alpha and PPAR-delta are primarily involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and anti-diabetic thiazolidinediones and affects the expression of genes involved in the storage of fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.Synonyms: NR1C3, PPARG, Peroxisome proliferator-activated receptor gamma
- 基因ID
- 5468
- NCBI登录号
- NP_005028
- UniProt
- P37231
- 途径
- MAPK Pathway, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
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