GFP 抗体 (HRP)
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北京 101111
Quick Overview for GFP 抗体 (HRP) (ABIN117894)
抗原
See all GFP 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This product was prepared from tissue culture supernatant by Protein A chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Peroxidase and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. Reactivity is observed against wild type, recombinant and enhanced forms of GFP.
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纯化方法
- Protein A chromatography
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免疫原
- The immunogen is a GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria
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亚型
- IgG2a
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anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IF, IP, FACS 宿主: 小鼠 Monoclonal 6C11C11 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IP, IC, ChIP-seq 宿主: 大鼠 Monoclonal 1A5 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IHC, IP, ICC, FACS 宿主: 小鼠 Monoclonal 4B10B2 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IF, IHC (fro), IHC (p), EM 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IHC, IP, FACS, FM, DB 宿主: 小鼠 Monoclonal 9F9-F9 unconjugated
anti-Green Fluorescent Protein (GFP) (AA 1-100) antibodyGFP 适用: Aequorea victoria WB, IF 宿主: 小鼠 Monoclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibody (DyLight 550)GFP 适用: Aequorea victoria WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 550
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Designed to detect GFP and its variants in ELISA (sandwich or capture), Immunoblottingand Immunoprecipitation. Monoclonal and polyclonal forms of anti-GFP assayed by ELISAfor direct binding of antigen recognize wild type, recombinant and enhanced forms of GFP. Monoclonal and polyclonal forms anti-GFP assayed in a sandwich ELISA are well suited totitrate GFP in solution using either form of the antibody as the capture or detection
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限制
- 仅限研究用
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溶解方式
- Restore with 1.0 mL of deionized water (or equivalent).
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Thimerisol as preservative and 10 mg/mL Bovine Serum Albumin (BSA) (IgG and Protease free) as stabilizer
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
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储存条件
- 4 °C/-20 °C
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储存方法
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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: "DNA Integration in Leishmania Genome: An Application for Vaccine Development and Drug Screening." in: Methods in molecular biology (Clifton, N.J.), Vol. 1403, pp. 603-22, (2016) (PubMed).
: "Dynamic in vivo interaction of DDB2 E3 ubiquitin ligase with UV-damaged DNA is independent of damage-recognition protein XPC." in: Journal of cell science, Vol. 120, Issue Pt 15, pp. 2706-16, (2007) (PubMed).
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- GFP (Green Fluorescent Protein (GFP))
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别名
- GFP
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背景
- Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y, antibodies raised against full-length GFP should also detect YFP and other variants.Synonyms: GFP-Tag, Green fluorescent protein
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基因ID
- 6100
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UniProt
- P42212
抗原
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