(4 references)-
- 抗原 See all IL12 抗体
- IL12 (Interleukin 12 (IL12))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This IL12 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Neutralization (Neut), Immunoprecipitation (IP)
- 品牌
- BD Pharmingen™
- 产品特性
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Neutralization Activity: This antibody has been reported to be useful for the neturalization of recombinant mouse IL-12 (p70). Neutralization activity may be measured with a proliferation assay using 300 pg/mL recombinant mouse IL-12 (p70) (Cat. No. 554592) to stimulate 2D6 cells at 1x10^5 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant mouse IL-12 (p70) for 60 minutes).
50% Neutralization (ND50) at 4 - 100 ng/mL
>95% Neutralization at 60 - 600 ng/mL - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
- 免疫原
- Recombinant Mouse IL-12 p70
- 克隆位点
- C17-8
- 亚型
- IgG2a
- Top Product
- Discover our top product IL12 Primary Antibody
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anti-Interleukin 12 (IL12) (AA 51-150) antibody
IL12 适用: 人 WB, ELISA, IF (p), IF (cc), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 (IL12) (AA 168-195) antibodyIL12 适用: 人 WB, ELISA, FACS, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 (IL12) antibodyIL12 适用: 小鼠 WB, ELISA, Neut 宿主: 山羊 Polyclonal unconjugated
anti-Interleukin 12 (IL12) antibodyIL12 适用: 人 WB, ELISA, Neut 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
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ELISA (IL-12 p70 Detection): The biotinylated C17.8 antibody (Cat. No. 554476) is useful as a detection antibody for a sandwich ELISA for specifically measuring mouse IL-12 p70 protein levels. Biotinylated C17.8 antibody can be paired with the purified 9A5 (Cat. No. 554658) as the capture antibody and with recombinant mouse IL-12 p70 (Cat. No.554592) as the standard. For testing mouse IL-12 p70 in complex biological fluids, such as serum or plasma, investigators may wish to consider using the Mouse IL-12 (p70) BD OptEIA™ ELISA Set (Cat. No. 555256).
ELISA (IL-12 p40 Detection): The biotinylated C17.8 antibody (Cat. No. 554476) is useful as a detection antibody for a sandwich ELISA measuring IL-12 p40 protein levels (free p40 monomer as well as p40 complexes as homodimer or heterodimer). Biotinylated C17.8 antibody can be paired with the purified C15.6 antibody (Cat. No. 551219) as the capture antibody, and with recombinant mouse IL-12 p70 protein (Cat. No.554592) or IL-12 p40 protein (Cat. No. 554594) as the standard. For testing mouse IL-12 p40 in complex biological fluids, such as serum or plasma, investigators may wish to consider using the Mouse IL-12 (p40) BD OptEIA™ ELISA Set (Cat. No. 555165). - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
- 储存液
- Azide free
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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: "Establishment of an IL-12-responsive T cell clone: its characterization and utilization in the quantitation of IL-12 activity." in: Journal of leukocyte biology, Vol. 61, Issue 3, pp. 346-52, (1997) (PubMed).
: "Antibodies to interleukin 12 abrogate established experimental colitis in mice." in: The Journal of experimental medicine, Vol. 182, Issue 5, pp. 1281-90, (1995) (PubMed).
: "Interleukin-12 is required for interferon-gamma production and lethality in lipopolysaccharide-induced shock in mice." in: European journal of immunology, Vol. 25, Issue 3, pp. 672-6, (1995) (PubMed).
: "Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells." in: The Journal of experimental medicine, Vol. 176, Issue 5, pp. 1387-98, (1992) (PubMed).
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: "Establishment of an IL-12-responsive T cell clone: its characterization and utilization in the quantitation of IL-12 activity." in: Journal of leukocyte biology, Vol. 61, Issue 3, pp. 346-52, (1997) (PubMed).
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- 抗原
- IL12 (Interleukin 12 (IL12))
- 别名
- IL-12 (p40/p70) (IL12 产品)
- 别名
- CLMF antibody, CLMF2 antibody, IL-12B antibody, NKSF antibody, NKSF2 antibody, p40 antibody, Il-12b antibody, Il12p40 antibody, Il-12p40 antibody, Il12 antibody, IL-12p40 antibody, IL-12 antibody, LOC100217488 antibody, IL-12p35 antibody, Il-12a antibody, Ll12a antibody, p35 antibody, IL12p40 antibody, IL12B antibody, IL-12 p35 antibody, interleukin 12B antibody, interleukin 12A antibody, interleukin 12b antibody, interleukin 12a antibody, Interleukin 12 level antibody, IL12B antibody, IL12A antibody, Il12b antibody, Il12a antibody, IL12 antibody, il12a antibody
- 背景
- The C17.8 antibody reacts with the free p40 monomer of IL-12 as well as the p40 subunit complexed as homodimer or complexed with p35 as a p70 heterodimer. Clone C17.8 has also been shown to be cross-reactive with mouse IL-23, which also contains the p40 subunit. The immunogen used to generate C17.8 hybridoma was recombinant mouse IL-12 p70.
- 途径
- JAK/STAT Signaling, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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