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Src 抗体 (pTyr418) (Alexa Fluor 488)

Name: Src 抗体 (pTyr418) (Alexa Fluor 488)
SKU: ABIN1177181
Availability: OutOfStock

This anti-Src antibody is a 小鼠单克隆 antibody detecting Src in ICS. Suitable for 人, 小鼠, 大鼠和小鸡. This Primary Antibody has been cited in 3+ publications.

(3 references)

产品编号 ABIN1177181

发货至: 中国

Quick Overview for Src 抗体 (pTyr418) (Alexa Fluor 488) (ABIN1177181)

抗原

See all Src 抗体

Src (Proto-oncogene tyrosine-protein kinase Src (Src))

适用

人, 小鼠, 大鼠, 小鸡

宿主

小鼠

克隆类型

单克隆

标记

This Src antibody is conjugated to Alexa Fluor 488

应用范围

Intracellular Staining (ICS)

克隆位点

K98-37

Discover our top product Src Primary Antibody

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pTyr418

品牌

BD Phosflow™

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Phosphorylated Human Src Peptide

亚型

IgG1 kappa
anti-Proto-oncogene tyrosine-protein kinase Src (Src) antibody
Src 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 1F11 unconjugated

anti-Proto-oncogene tyrosine-protein kinase Src (Src) (pTyr530) antibody
Src 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Proto-oncogene tyrosine-protein kinase Src (Src) (AA 1-80) antibody
Src 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Proto-oncogene tyrosine-protein kinase Src (Src) antibody
Src 适用: 人, 小鼠 WB, IF 宿主: 小鼠 Monoclonal 17AT28 unconjugated

anti-Proto-oncogene tyrosine-protein kinase Src (Src) (AA 24-52), (N-Term) antibody
Src 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB41141 unconjugated

anti-Proto-oncogene tyrosine-protein kinase Src (Src) antibody
KO Validated Src 适用: 人 WB, IF, IP 宿主: 兔 Monoclonal unconjugated

anti-Proto-oncogene tyrosine-protein kinase Src (Src) (AA 24-52) antibody
Src 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated

anti-Proto-oncogene tyrosine-protein kinase Src (Src) (AA 397-424) antibody
Src 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Proto-oncogene tyrosine-protein kinase Src (Src) (pTyr529) antibody
Src 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Proto-oncogene tyrosine-protein kinase Src (Src) (AA 1-90) antibody
Src 适用: 人 WB, ELISA, IF, IHC (p), PLA 宿主: 小鼠 Monoclonal 1B9 unconjugated

应用备注

Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation. Any of the three BD™ Phosflow permeabilization buffers may be used.

样本量

20 μL

限制

仅限研究用
状态

Liquid

缓冲液

Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Alvarez, Kantarjian, Cortes: "The role of Src in solid and hematologic malignancies: development of new-generation Src inhibitors." in: Cancer, Vol. 107, Issue 8, pp. 1918-29, (2006) (PubMed).

Frame: "Newest findings on the oldest oncogene; how activated src does it." in: Journal of cell science, Vol. 117, Issue Pt 7, pp. 989-98, (2004) (PubMed).

Arias-Salgado, Lizano, Sarkar, Brugge, Ginsberg, Shattil: "Src kinase activation by direct interaction with the integrin beta cytoplasmic domain." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 23, pp. 13298-302, (2003) (PubMed).
抗原

Src (Proto-oncogene tyrosine-protein kinase Src (Src))

别名

Src

背景

The non-receptor protein tyrosine kinases of the Src family have similar domain structures with considerable sequence homologies. They are associated with cell membranes and are activated by a wide variety of cell-surface receptors. Through their participation in cell-signaling cascades that mediate an array of cellular responses, they control essential cellular activities. Src, the prototype of the Src family, is encoded by the SRC protooncogene, which is a homolog of the v-Src gene of the Rous sarcoma virus. Src’s oncogenic potential may be a consequence of its role as a regulator of cell growth, morphology, motility, and adhesion. The protein contains domains that mediate protein-protein interactions and regulation of its tyrosine kinase activity. Mechanisms that regulate Src activity include phosphorylations of serines and tyrosines in most of the domains and interactions with receptor proteins, integrins, and other binding proteins. In particular, autophosphorylation at tyrosine 418 (Y418) activates tyrosine kinase activity. Phosphorylations at other sites are mediated by kinases such as cdc2, CSK, and CHK in signaling cascades and may up- or down-regulate Src activity. The K98-37 monoclonal antibody recognizes the phosphorylated Y418 in the protein kinase domain of activated Src. The orthologous phosphorylation sites in chicken, mouse, and rat Src are Y415, Y423, and Y418, respectively. Because of sequence similarities in this region, it may also recognize the homologous sites of some other Src family members: Lyn (pY396), Fyn (pY420), Hck (pY410), Lck (pY394), and Yes (pY425).
Synonyms: c-Src, p60-Src, SRC, SRC1

途径

JAK/STAT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling