我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

BCAR1 抗体 (pTyr249) (Alexa Fluor 647)

Name: BCAR1 抗体 (pTyr249) (Alexa Fluor 647)
SKU: ABIN1177115
Availability: OutOfStock

This anti-BCAR1 antibody is a 小鼠单克隆 antibody detecting BCAR1 in ICS. Suitable for 人. This Primary Antibody has been cited in 2+ publications.

(2 references)

产品编号 ABIN1177115

发货至: 中国

Quick Overview for BCAR1 抗体 (pTyr249) (Alexa Fluor 647) (ABIN1177115)

抗原

See all BCAR1 抗体

BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This BCAR1 antibody is conjugated to Alexa Fluor 647

应用范围

Intracellular Staining (ICS)

克隆位点

J169-757-12-2

Discover our top product BCAR1 Primary Antibody

抗原表位
30

22

15

6

5

4

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pTyr249

品牌

BD Phosflow™

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Phosphorylated Human p130Cas

亚型

IgG2b kappa
anti-Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) (AA 1-240) antibody
BCAR1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) antibody
BCAR1 适用: 人, 大鼠, 犬, 猴 WB, IHC, IHC (p), IF, FACS 宿主: 小鼠 Monoclonal 1B1 unconjugated

anti-Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) antibody
BCAR1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IHC (p), IF, FACS 宿主: 小鼠 Monoclonal 3A5 unconjugated

anti-Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) (AA 68-218) antibody
BCAR1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) (pTyr410) antibody
BCAR1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) (Internal Region) antibody
BCAR1 适用: 人, 小鼠 WB, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) (Tyr410) antibody
BCAR1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) (pTyr410) antibody
BCAR1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) (Internal Region) antibody
Verified BCAR1 适用: 人 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated

anti-Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) (AA 1-870) antibody
BCAR1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

样本量

20 μL

限制

仅限研究用
状态

Liquid

缓冲液

Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Goldberg, Alexander, Pellicena, Zhang, Tsuda, Miller: "Src phosphorylates Cas on tyrosine 253 to promote migration of transformed cells." in: The Journal of biological chemistry, Vol. 278, Issue 47, pp. 46533-40, (2003) (PubMed).

Hinsby, Olsen, Bennett, Mann: "Signaling initiated by overexpression of the fibroblast growth factor receptor-1 investigated by mass spectrometry." in: Molecular & cellular proteomics : MCP, Vol. 2, Issue 1, pp. 29-36, (2003) (PubMed).
抗原

BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

别名

p130 Cas

背景

P47v-crk (v-Crk) is the product of a transforming gene, v-crk, that was isolated from avian sarcoma viruses. The v-Crk protein is a fusion product of viral Gag protein and a part of cellular Crk that includes SH2 and SH3 domains. v-Crk-induced transformation increases tyrosine phosphorylation of several cellular proteins, including p130Cas (CRK-associated substrate). The p130Cas is tightly associated with v-Crk via the SH2 domain of v-Crk. Tyrosine phosphorylation of p130Cas occurs in conjunction with cellular transformation in cells that express v-Src or v-Crk. This phosphorylation leads to a change in p130Cas localization from the cytoplasm to the cell membrane and, possibly, to the nucleus. Since p130Cas also associates with v-Src, it may be a v-Src substrate. Several phosphorylation sites have been described in p130Cas upon Fibroblast Growth Factor stimulation, and phosphorylated tyrosine (Y249) might function as a binding site for the Crk-adaptor molecule. The J169-757.12.2 monoclonal antibody recognizes the phosphorylated Y249 of human p130Cas. The orthologous phosphorylation sites in mouse and rat p130Cas are Y253 and Y347, respectively.

途径

EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling