BCAR1 抗体 (pTyr249) (Alexa Fluor 647)
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北京 101111
Quick Overview for BCAR1 抗体 (pTyr249) (Alexa Fluor 647) (ABIN1177115)
抗原
See all BCAR1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pTyr249
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品牌
- BD Phosflow™
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纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Phosphorylated Human p130Cas
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亚型
- IgG2b kappa
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anti-Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) (AA 1-240) antibody
BCAR1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) antibodyBCAR1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IHC (p), IF, FACS 宿主: 小鼠 Monoclonal 3A5 unconjugated
anti-Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) antibodyBCAR1 适用: 人, 大鼠, 犬, 猴 WB, IHC, IHC (p), IF, FACS 宿主: 小鼠 Monoclonal 1B1 unconjugated
anti-Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) (AA 68-218) antibodyBCAR1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) (pTyr410) antibodyBCAR1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) (Internal Region) antibodyBCAR1 适用: 人, 小鼠 WB, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) (Tyr410) antibodyBCAR1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) (pTyr410) antibodyKD Validated BCAR1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB3870 unconjugated Recombinant Antibody
anti-Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) (pTyr410) antibodyBCAR1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) (Internal Region) antibodyVerified BCAR1 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
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样本量
- 20 μL
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "Src phosphorylates Cas on tyrosine 253 to promote migration of transformed cells." in: The Journal of biological chemistry, Vol. 278, Issue 47, pp. 46533-40, (2003) (PubMed).
: "Signaling initiated by overexpression of the fibroblast growth factor receptor-1 investigated by mass spectrometry." in: Molecular & cellular proteomics : MCP, Vol. 2, Issue 1, pp. 29-36, (2003) (PubMed).
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: "Src phosphorylates Cas on tyrosine 253 to promote migration of transformed cells." in: The Journal of biological chemistry, Vol. 278, Issue 47, pp. 46533-40, (2003) (PubMed).
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- BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))
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别名
- p130 Cas
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背景
- P47v-crk (v-Crk) is the product of a transforming gene, v-crk, that was isolated from avian sarcoma viruses. The v-Crk protein is a fusion product of viral Gag protein and a part of cellular Crk that includes SH2 and SH3 domains. v-Crk-induced transformation increases tyrosine phosphorylation of several cellular proteins, including p130Cas (CRK-associated substrate). The p130Cas is tightly associated with v-Crk via the SH2 domain of v-Crk. Tyrosine phosphorylation of p130Cas occurs in conjunction with cellular transformation in cells that express v-Src or v-Crk. This phosphorylation leads to a change in p130Cas localization from the cytoplasm to the cell membrane and, possibly, to the nucleus. Since p130Cas also associates with v-Src, it may be a v-Src substrate. Several phosphorylation sites have been described in p130Cas upon Fibroblast Growth Factor stimulation, and phosphorylated tyrosine (Y249) might function as a binding site for the Crk-adaptor molecule. The J169-757.12.2 monoclonal antibody recognizes the phosphorylated Y249 of human p130Cas. The orthologous phosphorylation sites in mouse and rat p130Cas are Y253 and Y347, respectively.
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途径
- EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling
抗原
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