beta Catenin 抗体 (AA 571-781) (Alexa Fluor 488)
(4 references)-
- 抗原 See all beta Catenin (CATNB) 抗体
- beta Catenin (CATNB) (Catenin, beta (CATNB))
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抗原表位
- AA 571-781
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适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This beta Catenin antibody is conjugated to Alexa Fluor 488
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应用范围
- Immunofluorescence (IF), BioImaging (BI), Intracellular Staining (ICS)
- 品牌
- BD Pharmingen™
- 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Mouse beta-Catenin aa. 571-781
- 克隆位点
- 14-Beta
- 亚型
- IgG1
- Top Product
- Discover our top product CATNB Primary Antibody
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anti-Catenin, beta (CATNB) (pSer33), (pSer37) antibody
CATNB 适用: 人, 小鼠, 大鼠 WB, FACS, ELISA, IF (cc), IF (p), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Catenin, beta (CATNB) (AA 661-781) antibodyCATNB 适用: 人, 小鼠, 大鼠, Pig, 斑马鱼 WB, FACS, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Catenin, beta (CATNB) (C-Term) antibodyCATNB 适用: 人, 小鼠, 大鼠, 小鸡, Cow, 犬, Pig, 兔, 绵羊, 猴, Hamster, Avian, Cat, 驴, 山羊, 豚鼠, 马 WB, IF 宿主: 山羊 Polyclonal unconjugated
anti-Catenin, beta (CATNB) (C-Term) antibodyCATNB 适用: 人, 斑马鱼 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Catenin, beta (CATNB) (pSer33), (pSer37), (pThr41) antibodyCATNB 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Catenin, beta (CATNB) (AA 439-675) antibodyCATNB 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Catenin, beta (CATNB) (AA 439-675) antibodyCATNB 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C901 unconjugated
anti-Catenin, beta (CATNB) antibodyCATNB 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 小鼠 Monoclonal 4A3H3 unconjugated
anti-Catenin, beta (CATNB) antibodyCATNB 适用: 人, 小鼠, Hamster WB, FACS, IP, ICC 宿主: 小鼠 Monoclonal EM-22 unconjugated
anti-Catenin, beta (CATNB) (AA 764-781) antibodyCATNB 适用: 人, 小鼠, 大鼠 WB, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
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- 样本量
- 5 μL
- 实验流程
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1. Seed ~10,000 cells in appropriate culture medium in a BD Falcon™ 96-well Imaging Plate (Cat. No. 353219) and culture overnight.
2. Remove the culture medium from the wells and fix the cells by adding 100 µl of fresh 3.7% Formaldehyde in PBS or BD Cytofix™ Fixation Buffer (Cat. No. 554655) to each well and incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either cold methanol or Triton™ X-100.
a. Add 100 myl of -20°C 90% methanol or -20°C BD Phosflow™ Perm Buffer III (Cat. No. 558050) to each well and incubate (5 min, RT). OR
b. Add 100 µl of 0.1% Triton™ X-100 (Sigma-Aldrich Cat. No. X-100) to each well and incubate (5 min, RT).
4. Remove the permeabilizer from the wells, and wash wells twice with 100 myl of 1x PBS.
5. Remove the PBS and block the cells by adding 100 myl of blocking buffer, eg, BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656) or 3% FBS in 1x PBS to each well and incubate (30 min, RT).
6. Meanwhile, put 5 ul (1 test size) Bioimaging Certified fluorescent antibodies into 45 ul of Stain Buffer, protect from light.
7. Remove the blocking buffer and add 50 myl of prepared fluorescent antibody per well and incubate (60 min, RT), protect from light.
8. Remove the fluorescent antibody and wash the wells three times with 100 myl of 1x PBS.
9. Remove the PBS and counterstain the nuclei by adding 100 µl of a 2 µg/ml solution of Hoechst 33342 (Cat. No. 561908) in 1x PBS to each well at least 15 minutes before imaging.
10. View and analyze the cells using an appropriate imaging instrument. - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
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: "A yeast model system for functional analysis of beta-catenin signaling." in: The Journal of cell biology, Vol. 158, Issue 6, pp. 1067-78, (2002) (PubMed).
: "Tumor suppressor PTEN inhibits nuclear accumulation of beta-catenin and T cell/lymphoid enhancer factor 1-mediated transcriptional activation." in: The Journal of cell biology, Vol. 153, Issue 6, pp. 1161-74, (2001) (PubMed).
: "The NEDD8 system is essential for cell cycle progression and morphogenetic pathway in mice." in: The Journal of cell biology, Vol. 155, Issue 4, pp. 571-9, (2001) (PubMed).
: "Uvomorulin-catenin complex formation is regulated by a specific domain in the cytoplasmic region of the cell adhesion molecule." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 11, pp. 4246-50, (1990) (PubMed).
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: "A yeast model system for functional analysis of beta-catenin signaling." in: The Journal of cell biology, Vol. 158, Issue 6, pp. 1067-78, (2002) (PubMed).
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- 抗原
- beta Catenin (CATNB) (Catenin, beta (CATNB))
- 别名
- beta-Catenin (CATNB 产品)
- 背景
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The 14/Beta-Catenin monoclonal antibody specifically binds to Beta-Catenin (beta-Catenin). beta-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions (zonula adherens). Deletions in the cytoplasmic domain of E-Cadherin which eliminate catenin binding also result in a loss of cell adhesion, indicating that this binding is essential for E-Cadherin function. Although the alpha- and beta-Catenins have been cloned, very little is known about their biochemical roles. However a link between beta-Catenin and colon cancer has been described. beta-Catenin was found to co-immunoprecipitate with the APC tumor suppressor protein in human colorectal tumor cell lines, as well as in human kidney 293 cells. E-Cadherin, however, was not detectable in these complexes. Thus the APC-Catenin complex may be affecting the transmission of contact inhibition signals and/or the regulation of cell adhesion.
Synonyms: CTNNB1, CTNB1, Catenin beta-1, catenin (cadherin-associated protein) beta 1 - 途径
- Peptide Hormone Metabolism
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