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14-3-3 sigma/SFN 抗体 (AA 1-248) (Biotin)

SFN 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal Biotin
产品编号 ABIN1176605
发货至: 中国
  • 抗原 See all 14-3-3 sigma/SFN (SFN) 抗体
    14-3-3 sigma/SFN (SFN) (Stratifin (SFN))
    抗原表位
    • 15
    • 12
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    • 9
    • 7
    • 7
    • 7
    • 6
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    • 3
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    AA 1-248
    适用
    • 102
    • 35
    • 28
    • 5
    • 5
    • 5
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    • 4
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    • 4
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    宿主
    • 92
    • 9
    • 2
    • 1
    克隆类型
    • 87
    • 17
    多克隆
    标记
    • 48
    • 10
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    • 4
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    • 3
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    This 14-3-3 sigma/SFN antibody is conjugated to Biotin
    应用范围
    • 85
    • 43
    • 37
    • 23
    • 17
    • 15
    • 14
    • 13
    • 13
    • 9
    • 3
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    Western Blotting (WB), Immunohistochemistry (IHC), ELISA
    序列
    MGHHHHHHSG SEF-MERASLIQKA KLAEQAERYE DMAAFMKGAV EKGEELSCEE RNLLSVAYKN VVGGQRAAWR VLSSIEQKSN EEGSEEKGPE VREYREKVET ELQGVCDTVL GLLDSHLIKE AGDAESRVFY LKMKGDYYRY LAEVATGDDK KRIIDSARSA YQEAMDISKK EMPPTNPIRL GLALNFSVFH YEIANSPEEA ISLAKTTFDE AMADLHTLSE DSYKDSTLIM QLLRDNLTLW TADNAGEEGG EAPQEPQS
    特异性
    It has been selected for its ability to recognize SFN in immunohistochemical staining and Western blotting.
    纯化方法
    Affinity Chromatography
    免疫原
    Recombinant SFN expressed in E.coli.
    The antibody is a rabbit polyclonal antibody raised against SFN conjugated to biotin.
    亚型
    IgG
    Top Product
    Discover our top product SFN Primary Antibody
  • 应用备注
    Western blotting: 1:100-400
    Immunocytochemistry in formalin fixed cells: 1:100-500
    Immunohistochemistry in formalin fixed frozen section: 1:100-500
    Immunohistochemistry in paraffin section: 1:50-200
    Enzyme-linked Immunosorbent Assay: 1:100-200
    Optimal working dilutions must be determined by end user.
    说明

    Content: The quality control contains recombinant SFN (Met1~Ser248) disposed in loading buffer.
    Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
    Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
    Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
    储存液
    Sodium azide
    注意事项
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    注意事项
    Avoid repeated freeze/thaw cycles
    储存条件
    4 °C
    储存方法
    Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
    有效期
    12 months
  • 抗原
    14-3-3 sigma/SFN (SFN) (Stratifin (SFN))
    Abstract
    SFN 产品
    别名
    YWHAS antibody, Er antibody, Mme1 antibody, Ywhas antibody, stratifin antibody, SFN antibody, Sfn antibody
    途径
    p53 Pathway, Myometrial Relaxation and Contraction
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