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Sonic Hedgehog 抗体 (AA 28-192)

This anti-Sonic Hedgehog antibody is a 兔 多克隆 antibody detecting Sonic Hedgehog in WB, IHC, IP 和 ICC. Suitable for 大鼠.
产品编号 ABIN1175088
发货至: 中国

Quick Overview for Sonic Hedgehog 抗体 (AA 28-192) (ABIN1175088)

抗原

See all Sonic Hedgehog (SHH) 抗体
Sonic Hedgehog (SHH)

适用

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大鼠

宿主

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克隆类型

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多克隆

标记

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This Sonic Hedgehog antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
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    AA 28-192

    原理

    Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH)

    特异性

    The antibody is a rabbit polyclonal antibody raised against SHH. It has been selected for its ability to recognize SHH in immunohistochemical staining and western blotting.

    纯化方法

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    免疫原

    RPB831Ra01Recombinant Hedgehog Homolog, Sonic (SHH)

    亚型

    IgG
  • 应用备注

    Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

    说明

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.27 mg/mL

    缓冲液

    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

    储存液

    ProClin

    注意事项

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    注意事项

    Avoid repeated freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    有效期

    12 months
  • 抗原

    Sonic Hedgehog (SHH)

    别名

    Hedgehog Homolog, Sonic

    背景

    HHG1, HLP3, HPE3, SMMCI, SHHN, SHH-N

    途径

    Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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