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EGF 抗体 (AA 45-275) (Biotin)

Name: EGF 抗体 (AA 45-275) (Biotin)
SKU: ABIN1173139
Availability: OutOfStock

EGF 适用: Pig WB, ELISA, IHC 宿主: 兔 Polyclonal Biotin

Western Blotting (WB) image for anti-Epidermal Growth Factor (EGF) (AA 45-275) antibody (Biotin) (ABIN1173139)

1 image

产品编号 ABIN1173139

发货至: 中国

Datasheet as PDF

抗原 See all EGF 抗体

EGF (Epidermal Growth Factor (EGF))
抗原表位
45

16

11

10

8

7

7

7

7

6

6

6

5

4

4

4

4

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 45-275
适用
152

85

50

12

10

5

4

1

1

1
Pig
宿主
216

30

9

3

1

1
兔
Compatible Secondaries
克隆类型
221

38
多克隆
标记
115

35

20

14

8

7

5

5

5

5

5

5

4

4

4

4

4

3

1

1

1

1

1

1

1

1
This EGF antibody is conjugated to Biotin
应用范围
206

86

61

53

53

29

25

24

17

16

15

14

8

8

4

4

3

2

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

序列

MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- APFLIF SHGNSIFRID LEGTNHEQLV ADAGISVLMD FHYNEERIYW VDLERQLLQR VFLNGTRQEK VCNLEKNVSG MAINWINEEL IWSNQQEGTI TVTDMKGNNS RVLLSALKYP ANVAVDPVER LMFWSSVVAG SLHRADVTGV EVRLLLETSE EIAAVSLDVL DKRLFWIQYN REGGSSRICS CDYDGGSVHF SKHLTQHNVF AMSLFGDHIF YSTWKKKTIW VANKH

特异性

It has been selected for its ability to recognize EGF in immunohistochemical staining and Western blotting.

纯化方法

Affinity Chromatography

免疫原

Recombinant EGF expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against EGF conjugated to biotin.

亚型

IgG

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应用备注

Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

说明

Content: The quality control contains recombinant EGF (Ala45~His275) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

储存液

Sodium azide

注意事项

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

注意事项

Avoid repeated freeze/thaw cycles

储存条件

4 °C

储存方法

Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

有效期

12 months
抗原

EGF (Epidermal Growth Factor (EGF))

Abstract

EGF 产品

别名

HOMG4 antibody, URG antibody, AI790464 antibody, CEGF antibody, epidermal growth factor antibody, pro-epidermal growth factor antibody, EGF antibody, egf antibody, CpipJ_CPIJ020278 antibody, Egf antibody

途径

NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation