CREB1 抗体 (pSer133)
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Quick Overview for CREB1 抗体 (pSer133) (ABIN116949)
抗原
See all CREB1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer133
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序列
- Q-K-R-R-E-I-L-S-R-R-P-pS-Y-R-K-I-L-N-D-L-S-S-D-A-P correspond ing to aa 122-147
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特异性
- This phospho specific polyclonal antibody reacts with phosphorylated human CREB at pS133 and shows minimal reactivity by western blot with non-phosphorylated CREB and minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first sequentially pre-adsorbed against a control E.coli lysate, nickel-purified recombinant CREB and the non-phosphorylated form of the immunizing peptide. The resultant depleted antiserum was then purified against the phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody is specific for phosphorylated pS133 of human CREB. Reactivity with non-phosphorylated CREB is minimal. This antibody does show cross reactivity with pS133 phosphorylated CREB from mouse and rat.
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纯化方法
- Affinity Purified
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免疫原
- CREB phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
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anti-cAMP Responsive Element Binding Protein 1 (CREB1) antibody
CREB1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 5G3 unconjugated
anti-cAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibodyCREB1 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-cAMP Responsive Element Binding Protein 1 (CREB1) (pSer142) antibodyCREB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-cAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibodyCREB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-cAMP Responsive Element Binding Protein 1 (CREB1) (AA 1-341) antibodyCREB1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-cAMP Responsive Element Binding Protein 1 (CREB1) (AA 1-95) antibodyCREB1 适用: 人 WB, ELISA, IHC (p), PLA 宿主: 小鼠 Monoclonal 5F2 unconjugated
anti-cAMP Responsive Element Binding Protein 1 (CREB1) (AA 1-341) antibodyCREB1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 3F1-1B2 unconjugated
anti-cAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibodyCREB1 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB05198 unconjugated
anti-cAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibodyCREB1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Monoclonal 3C11 unconjugated Recombinant Antibody
anti-cAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibodyCREB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: 1: 1,000. ELISA. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.064 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.1 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))
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别名
- CREB1
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背景
- The CREB (Cyclic AMP-response-element-binding-protein 1) gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element (CRE element TGANNTCA), an octameric palindrome. Phosphorylation by cAMPdependent protein kinase (PKA) at serine-119 is required for interaction with DNA and phosphorylation at serine-133 allows CREB to interact with CBP (CREB binding protein) leading to interaction with RNA polymerase II. Alternate splicing of this gene results in two transcript variants encoding different isoforms.Synonyms: CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element-binding protein 1
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基因ID
- 1385
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NCBI登录号
- NP_004370
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UniProt
- P16220
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途径
- TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation
抗原
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