IL-18 抗体
(2 references)-
- 抗原 See all IL-18 (IL18) 抗体
- IL-18 (IL18) (Interleukin 18 (IL18))
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适用
- 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This IL-18 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- This antibody is primarily directed against mature 18,000 MW mouse IL-18 and is useful in determining its presence in various assays. This antibody will also recognize the 24,000 inactive precursor form of Mouse IL-18. In general, this antibody also detects rat IL-18 in the same formats using similar dilutions.
- 纯化方法
- Protein A chromatography
- 免疫原
- The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with native 157 aa mouse IL-18 produced in E.coli.
- Top Product
- Discover our top product IL18 Primary Antibody
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anti-Interleukin 18 (IL18) (AA 37-194) antibody
IL18 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 18 (IL18) antibodyIL18 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 18 (IL18) (AA 37-193) antibodyIL18 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 18 (IL18) (AA 155-188), (C-Term) antibodyIL18 适用: 人 WB 宿主: 兔 Polyclonal RB48154 unconjugated
anti-Interleukin 18 (IL18) (AA 37-193) antibodyIL18 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C14 unconjugated
anti-Interleukin 18 (IL18) (Internal Region) antibodyVerified IL18 适用: 人 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Interleukin 18 (IL18) (AA 37-193) antibodyIL18 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 18 (IL18) (AA 36-192) antibodyIL18 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 18 (IL18) (AA 94-193) antibodyIL18 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C5 unconjugated
anti-Interleukin 18 (IL18) (AA 32-192) antibodyIL18 适用: 兔 WB, IHC, IP, ICC 宿主: 豚鼠 Polyclonal unconjugated
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- 应用备注
- This antibody is suitable for use in Neutralizations, ELISA, Immunohistochemistry andImmunoblotting. Although untested, this reagent may be useful for Radioimmunoassays,Flow cytometry and Immunoprecipitation. It recognizes the 18,000 MW mature (active)IL-18. Reactivity in other Immunoassays is unknown. Recommended Dilutions: This product has been assayed by immunoblot using HRP
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing no stabilizers or preservatives
- 储存液
- Azide free
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
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: "Identification of IL-18 and Th17 cells in salivary glands of patients with Sjögren's syndrome, and amplification of IL-17-mediated secretion of inflammatory cytokines from salivary gland cells by IL-18." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 4, pp. 2898-906, (2008) (PubMed).
: "Synthesis of naturally occurring iminosugars from D-fructose by the use of a zinc-mediated fragmentation reaction." in: Organic & biomolecular chemistry, Vol. 4, Issue 15, pp. 2898-905, (2006) (PubMed).
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: "Identification of IL-18 and Th17 cells in salivary glands of patients with Sjögren's syndrome, and amplification of IL-17-mediated secretion of inflammatory cytokines from salivary gland cells by IL-18." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 4, pp. 2898-906, (2008) (PubMed).
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- 抗原
- IL-18 (IL18) (Interleukin 18 (IL18))
- 别名
- Interleukin-18 / IL18 (IL18 产品)
- 别名
- Igif antibody, Il-18 antibody, IL-18 antibody, IGIF antibody, ChIL-18 antibody, IL-1g antibody, IL1F4 antibody, IL18 antibody, interleukin 18 antibody, Il18 antibody, IL18 antibody
- 背景
- Interleukin-18 (IL-18) is a member of the IL-1 cytokine family and was initially identified as an Interferon-g (IFN-g) inducing factor (IGIF). The IL-18 gene was originally cloned from liver cells and has since been shown to be produced by activated monocytes/ macrophages, Kupffer cells, keratinocytes, glucocorticoid-secreting adrenal cortex cells, osteoblasts and dendritic cells. IL-18 is a 24 kDa, non-glycosylated polypeptide that lacks a classical signal sequence and possesses a structure recognizably similar to IL-1. IL-18 is synthesized as a bio-inactive propeptide that undergoes proteolytic cleavage by either ICE (interleukin-1 beta converting enzyme) or another caspase to generate a mature, bioactive, 18 kDa molecule. In both the mature and propeptide forms, IL-18 shows 64 % aa sequence identity from mouse to human. IL-18 does not appear to show any primary sequence similarity to any other known cytokines. Rat IL-18 has also been isolated, and found to be 194 aa in length with a 91 % aa sequence identity to mouse IL-18. Human IL-18 has been found to induce the production of IFN-g and GM-CSF while inhibiting the production of IL-10 by PBMC. With respect to human T cells, IL-18 enhances Th1 cytokine production and stimulates cell proliferation via an IL-2-dependent pathway. Human IL-18 can also inhibit the synthesis of IgE by B cells. Thus, IL-18 plays an important role in immunological and inflammatory reactions. Currently, the bioactivity of human IL-18 is often determined by its capacity to augment the levels of IFN-g produced by T cells as measured in tissue culture supernatants.Synonyms: IGIF, IL-1 gamma, IL-18, IL1 gamma, IL1F4, Iboctadekin, Interferon-gamma-inducing factor, Interleukin-1 gamma
- 基因ID
- 16173
- NCBI登录号
- NP_032386
- UniProt
- P70380
- 途径
- Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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