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- 抗原 See all His Tag 抗体
- His Tag
- 适用
- 请咨询
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This His Tag antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
- 序列
- HHHHHH
- 纯化方法
- Affinity Chromatography.
- 免疫原
- 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide
- Top Product
- Discover our top product His Tag Primary Antibody
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anti-His Tag antibody
WB, ELISA, IP, IHC 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibodyWB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibodyWB, ELISA, IP, IHC, FACS 宿主: 小鼠 Monoclonal 33D10-D2-G8 unconjugated
anti-His Tag antibody适用: 人 WB 宿主: 小鼠 Monoclonal 6AT18 unconjugated
anti-His Tag antibodyWB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6G2A9 unconjugated
anti-His Tag antibody适用: 标记, 人 WB 宿主: 小鼠 Monoclonal 2D5 unconjugated
anti-His Tag antibody (HRP)WB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2 HRP
anti-His Tag (C-Term) antibodyWB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-His Tag (N-Term) antibody (Biotin)WB, ELISA, IP, FACS, ICC, IHC (p) 宿主: 兔 Monoclonal RM146 Biotin
anti-His Tag (N-Term) antibodyWB, IP, FACS, ICC, IHC (p) 宿主: 兔 Monoclonal RM146 unconjugated
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- 应用备注
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Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. This antibody has been tested by ELISA and western blotting against both the immunizingpeptide and His-containing recombinant proteins. Although not tested, this antibody islikely functional for Immunoprecipitation and Immunocytochemistry. Recommended Dilutions: ELISA: 1/10,000-1/40,000. Western blot: 1/1,000-1/3,000. Immunohistochemistry: 1/500-1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.
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- 抗原
- His Tag
- 别名
- 6xHistidine Epitope Tag (His Tag 产品)
- 物质类
- Tag
- 背景
- Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.
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