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Collagen Type I 抗体

Name: Collagen Type I 抗体
SKU: ABIN116549
Availability: OutOfStock

COL1 适用: 人, 大鼠, 小鼠, Cow, 哺乳动物 WB, IHC (fro), IHC (p), IP, EIA 宿主: 兔 Polyclonal unconjugated

Western blot analysis is shown using ABIN116549, affinity purified anti-Collagen I antibody to detect expression of collagen I in Wistar rat hepatic stellate cell (HSC) in control -GFP transduced (left lane) and -PPARgamma transduced cell lysates (right lane). (Collagen Type I 抗体)

1 image

(7 references)

产品编号 ABIN116549

发货至: 中国

Datasheet as PDF

抗原 See all Collagen Type I (COL1) 抗体

Collagen Type I (COL1) (Collagen, Type I (COL1))
适用
96

63

60

20

18

17

15

6

5

3

2

2

2

2

2

2

1
人, 大鼠, 小鼠, Cow, 哺乳动物
宿主
69

35

14
兔
Compatible Secondaries
克隆类型
83

35
多克隆
标记
59

14

7

6

3

3

3

3

3

3

3

3

3

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1
This Collagen Type I antibody is un-conjugated
应用范围
71

47

38

36

33

28

27

25

20

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9

6

6

5

4

1

1
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

特异性

This product has been prepared by Immunoaffinity Chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1 % cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I Collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other Human serum proteins or non-collagen extracellular matrix proteins is negligible.

纯化方法

Immunoaffinity Chromatography.

免疫原

Collagen type I purified from Human and Bovine placenta.

Top Product

Discover our top product COL1 Primary Antibody
anti-Collagen, Type I (COL1) antibody
COL1 适用: 人, 大鼠, 小鼠, Cow, 哺乳动物 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated

anti-Collagen, Type I (COL1) antibody
COL1 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated

anti-Collagen, Type I (COL1) (AA 1321-1400) antibody
COL1 适用: 人, 大鼠, 小鼠, 兔, 犬, 鱼 WB, ELISA, IHC (fro), IHC (p), IF (p), IF (cc), FACS 宿主: 兔 Polyclonal unconjugated

anti-Collagen, Type I (COL1) (AA 1051-1150) antibody
COL1 适用: 人, 大鼠, 小鼠, 兔 WB, ELISA, IHC (fro), IHC (p), IF (p), IF (cc), ICC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Collagen, Type I (COL1) antibody (FITC)
COL1 适用: 人, 大鼠, 小鼠, Cow, 哺乳动物 WB, IHC, IP, FACS 宿主: 兔 Polyclonal FITC

anti-Collagen, Type I (COL1) antibody (Biotin)
COL1 适用: 人, 大鼠, 小鼠, Cow, 哺乳动物 WB, ELISA, IHC, IP, FACS 宿主: 兔 Polyclonal Biotin

anti-Collagen, Type I (COL1) antibody (HRP)
COL1 适用: 人, 大鼠, 小鼠, Cow, 哺乳动物 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal HRP

anti-Collagen, Type I (COL1) antibody
COL1 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 小鼠 Monoclonal 3G3 unconjugated

anti-Collagen, Type I (COL1) antibody
COL1 适用: 人, 大鼠, 小鼠 IHC (p), IF 宿主: 小鼠 Monoclonal 4H10 unconjugated

anti-Collagen, Type I (COL1) antibody (Biotin)
COL1 适用: 人 ELISA 宿主: 山羊 Polyclonal Biotin

应用备注

Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation ofantigen in serum using a standard curve, for Immunoprecipitation and for native(non-denaturing, non-dissociating) PAGE and western blotting for highly sensitivequalitative analysis. Specific researchers have reported that this antibody is alsofunctional by conventional SDS-PAGE western blot. See references below for additionaldetails. Recommended Dilutions: ELISA: 1/5,000-1/50,000. Western blot: 1/500-1/5,000. Immunoprecipitation: 1/100. Immunohistochemistry: 1/50-1/200 (prepare tissues as frozen tissue sections).

限制

仅限研究用
浓度

1.0 mg/mL (by UV absorbance at 280 nm)

缓冲液

0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, 0.01 % (w/v) Sodium Azide, None

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

储存条件

4 °C/-20 °C

储存方法

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Yamanaka, Menuki, Tajima, Okada, Kosugi, Zenke, Sakai: "Effect of estradiol on fibroblasts from postmenopausal idiopathic carpal tunnel syndrome patients." in: Journal of cellular physiology, Vol. 233, Issue 11, pp. 8723-8730, (2018) (PubMed).

Caley, King, Shah, Wang, Rodriguez-Teja, Gronau, Waxman, Sturge: "Tumor-associated Endo180 requires stromal-derived LOX to promote metastatic prostate cancer cell migration on human ECM surfaces." in: Clinical & experimental metastasis, Vol. 33, Issue 2, pp. 151-65, (2016) (PubMed).

González-Miguel, Morchón, Siles-Lucas, Simón: "Fibrinolysis and proliferative endarteritis: two related processes in chronic infections? The model of the blood-borne pathogen Dirofilaria immitis." in: PLoS ONE, Vol. 10, Issue 4, pp. e0124445, (2015) (PubMed).

Carriel, Scionti, Campos, Roda, Castro, Cornelissen, Garzón, Alaminos: "In vitro characterization of a nanostructured fibrin agarose bio-artificial nerve substitute." in: Journal of tissue engineering and regenerative medicine, Vol. 11, Issue 5, pp. 1412-1426, (2015) (PubMed).

Nyström, Thriene, Mittapalli, Kern, Kiritsi, Dengjel, Bruckner-Tuderman: "Losartan ameliorates dystrophic epidermolysis bullosa and uncovers new disease mechanisms." in: EMBO molecular medicine, Vol. 7, Issue 9, pp. 1211-28, (2015) (PubMed).

Behrens, Villone, Koch, Brunner, Sorokin, Robenek, Bruckner-Tuderman, Bruckner, Hansen: "The epidermal basement membrane is a composite of separate laminin- or collagen IV-containing networks connected by aggregated perlecan, but not by nidogens." in: The Journal of biological chemistry, Vol. 287, Issue 22, pp. 18700-9, (2012) (PubMed).

Villone, Fritsch, Koch, Bruckner-Tuderman, Hansen, Bruckner: "Supramolecular interactions in the dermo-epidermal junction zone: anchoring fibril-collagen VII tightly binds to banded collagen fibrils." in: The Journal of biological chemistry, Vol. 283, Issue 36, pp. 24506-13, (2008) (PubMed).
抗原

Collagen Type I (COL1) (Collagen, Type I (COL1))

别名

Collagen Type I (COL1 产品)

别名

collagen type III alpha 1 chain antibody, COL3A1 antibody

背景

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens for immunization from human and bovine placenta and cartilage have been extensively purified by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS PAGE and immunoblotting.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2

基因ID

1277, 9606

UniProt

P02452