EGF 抗体
Quick Overview for EGF 抗体 (ABIN116365)
抗原
See all EGF 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Affinity chromatography
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免疫原
- Highly pure (>98%) recombinant mEGF
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anti-Epidermal Growth Factor (EGF) (AA 974-1026) antibody
EGF 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C11 unconjugated
anti-Epidermal Growth Factor (EGF) antibodyEGF 适用: 人 WB, DB 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor (EGF) antibodyEGF 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 9D7F11 unconjugated
anti-Epidermal Growth Factor (EGF) (AA 690-720), (Center) antibodyEGF 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB16555 unconjugated
anti-Epidermal Growth Factor (EGF) (AA 609-751) antibodyEGF 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor (EGF) antibodyEGF 适用: 小鼠 WB, ELISA, Neut 宿主: 山羊 Polyclonal unconjugated
anti-Epidermal Growth Factor (EGF) antibodyEGF 适用: 人 ELISA, ICC 宿主: 小鼠 Monoclonal 4E11 unconjugated
anti-Epidermal Growth Factor (EGF) (AA 974-1026) antibodyEGF 适用: 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor (EGF) (AA 1-30) antibodyEGF 适用: 小鼠 WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor (EGF) (AA 31-53) antibodyEGF 适用: 人 ELISA, IF (p), IF (cc), IHC (p), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Neutralization. ELISA: To detect mEGF by direct ELISA (using 100l/well antibody solution) a concentrationof at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/wellof recombinant mEGF. Western Blot: To detect mEGF by Western Blot analysis this antibody can be used at aconcentration of 0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mEGF is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- EGF (Epidermal Growth Factor (EGF))
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别名
- EGF
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背景
- Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane bound molecule which is proteolytically cleaved to generate the 53 amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein. Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.Synonyms: Epidermal growth factor, HOMG4, Pro-epidermal growth factor, URG, Urogastrone
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基因ID
- 10090
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UniProt
- P01132
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途径
- NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
抗原
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