IL12A 抗体
Quick Overview for IL12A 抗体 (ABIN116323)
抗原
See all IL12A 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Interleukin-12 (IL-12)
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纯化方法
- Affinity chromatography.
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免疫原
- Highly pure (>98%) recombinant mouse IL-12
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anti-Interleukin 12 alpha (IL12A) (AA 23-219) antibody
IL12A 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 alpha (IL12A) (AA 23-215) antibodyIL12A 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 alpha (IL12A) (AA 23-219) antibodyIL12A 适用: 人 WB 宿主: 小鼠 Monoclonal C3 unconjugated
anti-Interleukin 12 alpha (IL12A) (AA 23-215) antibodyIL12A 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 alpha (IL12A) (AA 51-150) antibodyIL12A 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 alpha (IL12A) antibodyMicroarray verified IL12A 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB517533 unconjugated
anti-Interleukin 12 alpha (IL12A) (AA 23-215) antibodyIL12A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 alpha (IL12A) (AA 23-219) antibodyIL12A 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 alpha (IL12A) antibodyMicroarray verified IL12A 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB224586 unconjugated
anti-Interleukin 12 alpha (IL12A) (AA 144-253) antibodyIL12A 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A6 unconjugated
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应用备注
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofmIL-12 (0.3 ng/mL), a concentration of 0.012 - 0.2 μg/mL of this antibody is required. ELISA: To detect mIL-12 by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant mIL-12. Western Blot: To detect mIL-12 alpha by Western Blot analysis this antibody can be used ata concentration of 0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mIL-12 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions. Immunohistochemistry: We recommend use of 1 g per ml as a starting dilution. Paraffinsections may require pretreatment like Proteinase K or Trypsin predigestion to improvestaining.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- IL12A (Interleukin 12 alpha (IL12A))
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别名
- Interleukin-12 alpha / IL12A
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背景
- Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. IL12 induces IFN gamma, TNF alpha production in T and NK cells, costimulation of PBL proliferation, proliferation/differentiation of TH1 T lymphocytes. It is downregulated by IL10, homodimeric p40 antagonistic to functional p70 heterodimer. IL12R beta 1 binds p40 but when dimeric with IL12R beta 2 binds p35.Synonyms: CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, IL-12A, NK cell stimulatory factor chain 1, NKSF1
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基因ID
- 16159
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NCBI登录号
- NP_032377
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UniProt
- P43431
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
抗原
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