VEGFA 抗体 (Biotin)
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Quick Overview for VEGFA 抗体 (Biotin) (ABIN116249)
抗原
See all VEGFA 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody reacts with Vascular Endothelial Growth Factor.
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纯化方法
- Affinity chromatography.
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免疫原
- Highly pure (>98%) recombinant hVEGF (human VEGF)
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应用备注
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ELISA: To detect hVEGF by direct ELISA (using 100 ml/well antibody solution) this antibodycan be used at a concentration of 0.15-0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hVEGF. Western Blot: To detect hVEGF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 mg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hVEGF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
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别名
- VEGF-A
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背景
- Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor
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基因ID
- 7422
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NCBI登录号
- NP_001020537
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UniProt
- P15692
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途径
- RTK signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
抗原
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