TGFA 抗体
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北京 101111
Quick Overview for TGFA 抗体 (ABIN116239)
抗原
See all TGFA 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody reacts with Transforming Growth Factor alpha.
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纯化方法
- Affinity chromatography.
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免疫原
- Highly pure (>98%) recombinant hTGF-alpha
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anti-Transforming Growth Factor, alpha (TGFA) (AA 40-89) antibody
TGFA 适用: 人, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Transforming Growth Factor, alpha (TGFA) (AA 24-98) antibodyTGFA 适用: 人 IHC, WB, IP, ICC 宿主: 小鼠 Monoclonal D20 unconjugated
anti-Transforming Growth Factor, alpha (TGFA) (AA 24-98) antibodyTGFA 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transforming Growth Factor, alpha (TGFA) (AA 40-89) antibodyTGFA 适用: 人 IHC, WB, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Transforming Growth Factor, alpha (TGFA) antibodyTGFA 适用: 人, 大鼠, 小鼠 IHC, WB, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transforming Growth Factor, alpha (TGFA) antibodyTGFA 适用: 人, 大鼠, 小鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Transforming Growth Factor, alpha (TGFA) (AA 118-150) antibodyTGFA 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB56968 unconjugated
anti-Transforming Growth Factor, alpha (TGFA) (AA 1-160) antibodyTGFA 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Transforming Growth Factor, alpha (TGFA) (C-Term) antibodyTGFA 适用: 人, 大鼠, 小鼠, Cow IHC, IF, ELISA, StM, Coat 宿主: 小鼠 Monoclonal MF9 unconjugated
anti-Transforming Growth Factor, alpha (TGFA) antibodyTGFA 适用: 人 IHC, WB, ELISA, IHC (p), Neut 宿主: 兔 Polyclonal unconjugated
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应用备注
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhTGF-α (0.5 ng/mL), a concentration of 2.5-2.7 μg/mL of this antibody is required. ELISA: (Indirect): To detect hTGF-α by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hTGF-α. (Sandwich): To detect hTGF-α by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human TGF-α (PP1070B1 or PP1070B2) as adetection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTGF-α. Western Blot: To detect hTGF-α by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hTGF-α is 1.5- 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- TGFA (Transforming Growth Factor, alpha (TGFA))
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别名
- TGF-alpha (TGFA)
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背景
- Synonyms: EGF-like TGF, ETGF, TGF type 1, Transforming growth factor alpha
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基因ID
- 7039
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NCBI登录号
- NP_001093161
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UniProt
- P01135
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途径
- NF-kappaB Signaling, RTK signaling, EGFR Signaling Pathway
抗原
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