PDGFB 抗体
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- 抗原 See all PDGFB 抗体
- PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PDGFB antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)
- 特异性
- Recognizes Human PDGF-BB.
- 交叉反应 （详细）
- Species reactivity (tested):Human.
- 纯化方法
- Affinity Chromatography employing immobilized Human PDGF-BB matrix.
- 免疫原
- Highly pure (>98%) E.coli derived recombinant Human PDGF-BB.
- Top Product
- Discover our top product PDGFB Primary Antibody
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anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 30-110) antibody
PDGFB 适用: 人, 大鼠, 小鼠 WB, ELISA, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 20-241) antibodyPDGFB 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 155-210) antibodyPDGFB 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor Subunit B (PDGFB) antibodyPDGFB 适用: 人, 大鼠, 小鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 84-199) antibodyPDGFB 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 101-201) antibody (Biotin)PDGFB 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal Biotin
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 101-201) antibody (PE)PDGFB 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal PE
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 101-201) antibody (Atto 594)PDGFB 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal Atto 594
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 101-201) antibody (Atto 488)PDGFB 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal Atto 488
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 101-201) antibody (Atto 390)PDGFB 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal Atto 390
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- 应用备注
- Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman PDGF-BB (2.5 ng/mL), a concentration of 0.03-0.04 μg/mL of this antibody isrequired. ELISAIndirect: To detect Human PDGF-BB by indirect ELISA (using 100 μL/well antibody solution)a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant Human PDGF-BB. Sandwich: To detect Human PDGF-BB by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with Biotinylated Anti-Human PDGF-BB (PP1062B1 orPP1062B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant Human PDGF-BB. Western Blot: To detect Human PDGF-BB by Western Blot analysis this antibody can beused at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human PDGF-BB is 1.5-3.0 ng/lane, undereither reducing or non-reducing conditions.
- 限制
- 仅限研究用
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- 溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
- 缓冲液
- PBS, pH 7.2 without preservatives.
- 储存液
- Without preservative
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized antibody is stable at -20 °C for one year from despatch. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.
- 有效期
- 12 months
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- 抗原
- PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))
- 别名
- PDGFB (PDGFB 产品)
- 别名
- PDGF-2 antibody, PDGF2 antibody, SIS antibody, SSV antibody, c-sis antibody, pdgf2 antibody, sis antibody, si:ch211-79m20.1 antibody, si:dkey-261d11.1 antibody, PDGF-B antibody, Sis antibody, platelet derived growth factor subunit B antibody, platelet-derived growth factor beta polypeptide antibody, platelet derived growth factor subunit B S homeolog antibody, platelet-derived growth factor beta polypeptide a antibody, platelet derived growth factor, B polypeptide antibody, PDGFB antibody, pdgfb.S antibody, pdgfba antibody, Pdgfb antibody
- 背景
- Platelet Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimer composed of two related polypeptides designated A and B, the dimers being connected by disulfide bonds. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis.Synonyms: Becaplermin, PDGF B, PDGF beta, PDGF subunit B, PDGF-2, PDGF-B, PDGF2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta, Platelet-derived growth factor subunit B, SIS, c-Sis
- 基因ID
- 5155
- NCBI登录号
- NP_002599
- UniProt
- P01127
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
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