PDGFB 抗体
Quick Overview for PDGFB 抗体 (ABIN116211)
抗原
See all PDGFB 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes Human PDGF-BB.
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交叉反应 （详细）
- Species reactivity (tested):Human.
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纯化方法
- Affinity Chromatography employing immobilized Human PDGF-BB matrix.
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免疫原
- Highly pure (>98%) E.coli derived recombinant Human PDGF-BB.
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anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 20-241) antibody
PDGFB 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 155-210) antibodyPDGFB 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 84-199) antibodyPDGFB 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (C-Term) antibodyVerified PDGFB 适用: 人 ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 1-241) antibodyPDGFB 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (N-Term) antibodyPDGFB 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 82-190) antibodyPDGFB 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (N-Term) antibodyPDGFB 适用: 人, 大鼠, 小鼠, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 82-190) antibodyPDGFB 适用: 人, 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 21-241) antibodyPDGFB 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman PDGF-BB (2.5 ng/mL), a concentration of 0.03-0.04 μg/mL of this antibody isrequired. ELISAIndirect: To detect Human PDGF-BB by indirect ELISA (using 100 μL/well antibody solution)a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant Human PDGF-BB. Sandwich: To detect Human PDGF-BB by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with Biotinylated Anti-Human PDGF-BB (PP1062B1 orPP1062B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant Human PDGF-BB. Western Blot: To detect Human PDGF-BB by Western Blot analysis this antibody can beused at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human PDGF-BB is 1.5-3.0 ng/lane, undereither reducing or non-reducing conditions.
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限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized antibody is stable at -20 °C for one year from despatch. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.
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有效期
- 12 months
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- PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))
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别名
- PDGFB
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背景
- Platelet Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimer composed of two related polypeptides designated A and B, the dimers being connected by disulfide bonds. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis.Synonyms: Becaplermin, PDGF B, PDGF beta, PDGF subunit B, PDGF-2, PDGF-B, PDGF2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta, Platelet-derived growth factor subunit B, SIS, c-Sis
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基因ID
- 5155
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NCBI登录号
- NP_002599
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UniProt
- P01127
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
抗原
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