PDGFB 抗体 (Biotin)
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- 抗原 See all PDGFB 抗体
- PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PDGFB antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- Recognizes Human Platelet Derived Growth Factor BB (hPDGF-BB)
- 纯化方法
- Affinity Chromatography.
- 免疫原
- Highly pure (>98%) Recombinant Human PDGF-B.
- Top Product
- Discover our top product PDGFB Primary Antibody
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anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 20-241) antibody
PDGFB 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 155-210) antibodyPDGFB 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 84-199) antibodyPDGFB 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 101-201) antibody (Biotin)PDGFB 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal Biotin
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 101-201) antibody (PE)PDGFB 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal PE
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 101-201) antibody (Atto 594)PDGFB 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal Atto 594
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 101-201) antibody (Atto 488)PDGFB 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal Atto 488
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 101-201) antibody (Atto 390)PDGFB 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal Atto 390
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (AA 1-241) antibodyPDGFB 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Platelet Derived Growth Factor Subunit B (PDGFB) (N-Term) antibodyPDGFB 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Direct ELISA: To detect Human PDGF-BB by direct ELISA (using 100 μL/well antibodysolution) this antibody can be used at a concentration of approx. 1.0 μg/mL. This Biotinylatedantibody allows the detection of at least 0.2 ng/well of recombinant hPDGF-BB. Sandwich ELISA: To detect Human PDGF-BB by Sandwich ELISA (using 100 μL/well antibodysolution) this antibody can be used at a concentration of 0.25-1.0 μg/mL. This Biotinylatedantibody, in conjunction with anti-Human PDGF-BB antibody (PP1062P1 or PP1062P2) as aCapture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinanthPDGF-BB. Western Blot: To detect hPDGF-BB by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hPDGF-BB is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
- 缓冲液
- PBS, pH 7.2 without preservatives.
- 储存液
- Without preservative
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- 抗原
- PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))
- 别名
- PDGFB (PDGFB 产品)
- 背景
- Platelet Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimer composed of two related polypeptides designated A and B, the dimers being connected by disulfide bonds. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis.Synonyms: Becaplermin, PDGF B, PDGF beta, PDGF subunit B, PDGF-2, PDGF-B, PDGF2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta, Platelet-derived growth factor subunit B, SIS, c-Sis
- 基因ID
- 5155
- NCBI登录号
- NP_002599
- UniProt
- P01127
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
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