PDGFA 抗体
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北京 101111
Quick Overview for PDGFA 抗体 (ABIN116207)
抗原
See all PDGFA 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- The antibody recognizes the human Platelet Derived Growth Factor AA (hPDGF-AA). Not tested in other species.
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纯化方法
- Immunoaffinity chromatography using immobilized human PDGF matrix
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免疫原
- Highly pure (>98%) recombinant hPDGF-AA
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anti-Platelet Derived Growth Factor A (PDGFA) (AA 125-170) antibody
PDGFA 适用: 人, 小鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor A (PDGFA) (AA 1-211) antibodyPDGFA 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor A (PDGFA) antibodyPDGFA 适用: 人, 小鼠, 大鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor A (PDGFA) (AA 1-211) antibodyPDGFA 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor A (PDGFA) antibodyPDGFA 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor A (PDGFA) (AA 84-193) antibodyPDGFA 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Platelet Derived Growth Factor A (PDGFA) (AA 1-211) antibodyPDGFA 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Platelet Derived Growth Factor A (PDGFA) (Center) antibodyPDGFA 适用: 人, 小鼠, 大鼠, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Platelet Derived Growth Factor A (PDGFA) (AA 60-89), (N-Term) antibodyPDGFA 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB01999 unconjugated
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应用备注
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Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity of hPDGF-AA (5.0 ng/mL),a concentration of 0.05 - 0.07 μg/mL of this antibody is required. Immunohistochemistry: The antibody stained formalin-fixed, paraffin embedded sections of normal human skin at1.0 μg/mL overnight at 4C. (A HRP-labeled polymer detection system was used with anon-alcohol soluble AEC chromogen and a proteinase K antigen retrieval). ELISA: Indirect - To detect hPDGF-AA by indirect ELISA (using 100 μL/well antibody solution),a concentration of 0.5 - 2.0 μg/mL of this antibody is required, allows the detection of atleast 0.2 - 0.4 ng/well of recombinant hPDGF-AA. Sandwich - To detect hPDGF-AA by sandwich ELISA (using 100 μL/well antibody solution),a concentration of 0.5 - 2.0 μg/mL of this antibody is required, in conjunction with abiotinylated anti-human PDGF-AA (PP1061B) as the detection antibody at least 0.2 - 0.4 ng/well of recombinant hPDGF-AA can be detected. Western blot: To detect hPDGF-AA by Western blot analysis this antibody can be used at a concentrationof 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hPDGF-AA is 1.5 -3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2, without preservatives
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized antibody is stable at -20 °C for one year from despatch. The reconstituted antibody can be stored for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.
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有效期
- 12 months
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- PDGFA (Platelet Derived Growth Factor A (PDGFA))
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别名
- PDGFA
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背景
- Synonyms: PDGF A, PDGF alpha, PDGF subunit A, PDGF-1, PDGF-A, PDGF1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha, Platelet-derived growth factor subunit A
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基因ID
- 5154
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NCBI登录号
- NP_002598
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UniProt
- P04085
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
抗原
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