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Interleukin 17a 抗体

IL17A 适用: 人 WB, EIA, IHC (p), Func 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN116119
发货至: 中国
  • 抗原 See all Interleukin 17a (IL17A) 抗体
    Interleukin 17a (IL17A) (Interleukin 17A (IL17A))
    适用
    • 89
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    宿主
    • 61
    • 40
    • 17
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    克隆类型
    • 70
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    多克隆
    标记
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    This Interleukin 17a antibody is un-conjugated
    应用范围
    • 71
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    Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)
    特异性
    This antibody reacts with Interleukin-17 (IL-17).
    纯化方法
    Affinity Chromatography.
    免疫原
    Highly pure (>98%) recombinant hIL-17A (human Interleukin-17A).
    Top Product
    Discover our top product IL17A Primary Antibody
  • 应用备注
    Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman IL-17A (50.00 ng/mL), a concentration of 0.9-1.3 μg/mL of previous lots of thisantibody is required. Immunohistochemistry on Parrafin Sections: 0.25 μg/mL, Overnight at 4 °C. This antibody stained sections of Human breast invasive ductal carcinoma. An HRP-labeledpolymer detection system was used with a DAB Chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Sandwich ELISA: To detect Human IL-17A by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with Biotinylated anti-Human IL-17A (PP1037B1 orPP1037B2) as Detection antibody, allows the detection of 0.2-0.4 ng/well of recombinantHuman IL-17A. Western Blot: To detect Human IL-17A by Western Blot analysis this antibody can be usedat a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human IL-17A is 1.5-3.0 ng/lane, under either
    限制
    仅限研究用
  • 溶解方式
    Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
    缓冲液
    PBS, pH 7.2 without preservatives.
    储存液
    Without preservative
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody at 2-8 °C for one month or at -20 °C for longer. This product should be stored undiluted.
  • 抗原
    Interleukin 17a (IL17A) (Interleukin 17A (IL17A))
    别名
    Interleukin-17A (IL17A) (IL17A 产品)
    别名
    CTLA8 antibody, IL-17 antibody, IL-17A antibody, IL17 antibody, Ctla-8 antibody, Ctla8 antibody, Il17 antibody, ChIL-17 antibody, IL-17F antibody, IL17A antibody, CTLA-8 antibody, interleukin 17A antibody, IL17A antibody, Il17a antibody
    背景
    IL17, also known as CTLA8, is a T cell derived hematopoietic cytokine. It was originally cloned from a T cell hybridoma produced by fusion of a mouse cytotoxic T cell clone and a rat T lymphoma. IL17 exhibits multiple biological activities on a variety of cells including: the induction of IL6, IL8 and GCSF production in fibroblasts, the enhancement of surface expression of ICAM 1 in fibroblasts, activation of NF kappaB and costimulation of T cell proliferation. IL17 is an approximately 16 kDa polypeptide of 136 amino acids. The precursor form of IL17 consists of 155 amino acids. To generate the mature IL17 (136 amino acids), the precursor cleaves a 19 amino acid signal peptide. Human IL17 shows approximately 62.5 % amino acid homology to mouse IL17 and 58 % amino acid homology to rat IL17.Synonyms: CTLA-8, CTLA8, Cytotoxic T-lymphocyte-associated antigen 8, IL-17, IL-17A, IL17
    基因ID
    3605
    NCBI登录号
    NP_002181
    UniProt
    Q16552
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