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Interleukin 17a 抗体

IL17A 适用: 人 ELISA, WB, Neut 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN465126
发货至: 中国
  • 抗原 See all Interleukin 17a (IL17A) 抗体
    Interleukin 17a (IL17A) (Interleukin 17A (IL17A))
    适用
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    宿主
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    山羊
    克隆类型
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    多克隆
    标记
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    This Interleukin 17a antibody is un-conjugated
    应用范围
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    ELISA, Western Blotting (WB), Neutralization (Neut)
    特异性
    Human IL-17A
    纯化方法
    Immunoaffinity purified
    免疫原
    E.coli derived recombinant Human IL-17A.

    Type of Immunogen: Cell extract
    Top Product
    Discover our top product IL17A Primary Antibody
  • 应用备注
    Approved: ELISA (0.5 - 2 μg/mL), Neut (0.43 - 0.65 μg/mL), WB

    Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.125 μg/mL-0.25 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-17A (50.00 ng/mL), a concentration of 0.43-0.65 μg/mL of this antibody is required. ELISA: To detect hIL-17A by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-17A (LS-C104417) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-17A. Western Blot: To detect hIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17A is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitute in sterile water
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS,  pH 7.2
    注意事项
    Avoid freeze/thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原
    Interleukin 17a (IL17A) (Interleukin 17A (IL17A))
    别名
    IL17A / IL17 (IL17A 产品)
    别名
    CTLA8 antibody, IL-17 antibody, IL-17A antibody, IL17 antibody, Ctla-8 antibody, Ctla8 antibody, Il17 antibody, ChIL-17 antibody, IL-17F antibody, IL17A antibody, CTLA-8 antibody, interleukin 17A antibody, IL17A antibody, Il17a antibody
    背景
    Name/Gene ID: IL17A
    Family: Interleukin

    Synonyms: IL17A, CTLA-8, CTLA8, Interleukin-17A, Interleukin 17A, IL-17, IL-17A, IL17
    基因ID
    3605
    UniProt
    Q16552
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