IL-8 抗体 (Biotin)
Quick Overview for IL-8 抗体 (Biotin) (ABIN116089)
抗原
See all IL-8 (IL8) 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Affinity chromatography.
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免疫原
- Highly pure (>98%) recombinant hIL-8 (human IL-8).
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anti-Interleukin 8 (IL8) (AA 23-101) antibody
IL8 适用: 马 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 8 (IL8) (AA 21-99) antibodyIL8 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 8 (IL8) antibodyIL8 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
anti-Interleukin 8 (IL8) (AA 72-99) antibodyIL8 适用: 人 WB, IHC, ELISA, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 8 (IL8) antibodyIL8 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 8 (IL8) (AA 72-99), (C-Term) antibodyIL8 适用: 人 WB, FACS, IHC (p), IF 宿主: 兔 Polyclonal RB20676 unconjugated
anti-Interleukin 8 (IL8) (AA 23-101) antibodyIL8 适用: 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 8 (IL8) (AA 23-101) antibodyIL8 适用: 犬 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C4 unconjugated
anti-Interleukin 8 (IL8) (AA 28-99) antibodyIL8 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 8 (IL8) (AA 23-99) antibodyIL8 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: To detect hIL-8 by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hIL-8. Western Blot: To detect hIL-8 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-8 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2 without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody at 4-8 °C for one month or at -20 °C for longer.
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- IL-8 (IL8) (Interleukin 8 (IL8))
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别名
- Interleukin-8 / IL8
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背景
- IL8 is a member of the CXC chemokine family. This family of small basic heparan-binding proteins are proinflammatory and primarily mediate the activation and migration of neutrophils into tissue from peripheral blood. This chemokine is one of the major mediators of the inflammatory response and is secreted by several cell types in response to an inflammatory stimulus. It functions as a chemoattractant, and is also a potent angiogenic factor. IL8 attracts neutrophils, basophils, and T-cells, but not monocytes. Cystic fibrosis (CF) is characterized by severe lung inflammation. The inflammatory process is believed to be caused by massive overproduction of the proinflammatory protein IL8, and the high levels of IL8 in the CF lung are therefore believed to be the central mechanism behind CF lung pathophysiology.Synonyms: CXCL8, Emoctakin, GCP1, Granulocyte chemotactic protein 1, MDNCF, MONAP, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, NAP1, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor
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基因ID
- 9606
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UniProt
- P10145
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途径
- TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy
抗原
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