IL12A 抗体
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- 抗原 See all IL12A 抗体
- IL12A (Interleukin 12 alpha (IL12A))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This IL12A antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)
- 特异性
- Detects Mouse IL-12. Other species not tested.
- 纯化方法
- Affinity Chromatography on Protein G
- 免疫原
- Recombinant murine IL-12
- 亚型
- IgG1
- Top Product
- Discover our top product IL12A Primary Antibody
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anti-Interleukin 12 alpha (IL12A) (AA 23-219) antibody
IL12A 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 alpha (IL12A) (AA 23-215) antibodyIL12A 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 alpha (IL12A) (AA 23-219) antibodyIL12A 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C9 unconjugated
anti-Interleukin 12 alpha (IL12A) (AA 23-215) antibodyIL12A 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 alpha (IL12A) (AA 51-150) antibodyIL12A 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 alpha (IL12A) antibodyMicroarray verified IL12A 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB517533 unconjugated
anti-Interleukin 12 alpha (IL12A) (AA 23-215) antibodyIL12A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 alpha (IL12A) (AA 23-219) antibodyIL12A 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 12 alpha (IL12A) antibodyMicroarray verified IL12A 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB224586 unconjugated
anti-Interleukin 12 alpha (IL12A) (AA 144-253) antibodyIL12A 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A6 unconjugated
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- 应用备注
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ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 3-4 μg/mL of thisantibody will detect at least 0.1 ng/well of recombinant murine IL-12 when used withbiotinylated antigen affinity purified anti-murine IL-12 as the detection antibody. Neutralisation: To yield one-half maximal inhibition (ND50) of the biological activity ofmurine IL-12 (2.0 ng/mL), a concentration of 0.16-0.24 μg/mL of this antibody is required. Western Blot: To detect mIL-12 by Western Blot analysis this antibody can be used at aconcentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mIL-12 is 1.0-2.0 ng/lane, under reducing conditionsand 0.25-0.50 ng/lane, under non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore in sterile water to a concentration of 1.0 mg/mL.
- 缓冲液
- PBS without preservatives
- 储存液
- Without preservative
- 注意事项
- Avoid repeated freezing and thawing. Protect from light!
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody at 2-8 °C under subdued light for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- IL12A (Interleukin 12 alpha (IL12A))
- 别名
- Interleukin-12 alpha / IL12A (IL12A 产品)
- 别名
- CLMF antibody, IL-12A antibody, NFSK antibody, NKSF1 antibody, P35 antibody, CLMF2 antibody, IL-12B antibody, NKSF antibody, NKSF2 antibody, p40 antibody, Il-12b antibody, Il12p40 antibody, Il-12p40 antibody, Il12 antibody, IL-12p40 antibody, IL-12p35 antibody, Il-12a antibody, Ll12a antibody, p35 antibody, IL12p35 antibody, Il-12 p35 antibody, IL12p40 antibody, IL12 antibody, IL-12 p35 antibody, interleukin 12A antibody, interleukin 12B antibody, interleukin 12b antibody, interleukin 12a antibody, Interleukin 12 level antibody, IL12A antibody, IL12B antibody, Il12b antibody, Il12a antibody, IL12 antibody, il12a antibody
- 背景
- Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. IL12 induces IFN gamma, TNF alpha production in T and NK cells, costimulation of PBL proliferation, proliferation/differentiation of TH1 T lymphocytes. It is downregulated by IL10, homodimeric p40 antagonistic to functional p70 heterodimer. IL12R beta 1 binds p40 but when dimeric with IL12R beta 2 binds p35.Synonyms: CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, IL-12A, NK cell stimulatory factor chain 1, NKSF1
- 基因ID
- 16159
- NCBI登录号
- NP_032377
- UniProt
- P43431
- 途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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