His Tag 抗体
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北京 101111
Quick Overview for His Tag 抗体 (ABIN115691)
抗原
See all His Tag 抗体宿主
克隆类型
标记
应用范围
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序列
- HHHHHH
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纯化方法
- Antigen Affinity Chromatography using a His-peptide as matrix.
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免疫原
- Different highly purified 6x His-tagged proteins (C-terminal) produced in insect cells.
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anti-His Tag antibody
适用: 人 WB 宿主: 小鼠 Monoclonal 6AT18 unconjugated
anti-His Tag antibodyWB, ELISA, IHC, DB 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibodyWB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibodyWB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6G2A9 unconjugated
anti-His Tag antibodyWB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2-G8 unconjugated
anti-His Tag antibody适用: 人 WB 宿主: 兔 Polyclonal RB19960 unconjugated
anti-His Tag antibodyWB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal HIS-H8 unconjugated
anti-His Tag antibody (DyLight 649)WB, ELISA, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 DyLight 649
anti-His Tag antibody (HRP)WB, ELISA, IHC 宿主: 小鼠 Monoclonal 33D10-D2-G8 HRP
anti-His Tag antibody (Cy3)WB, ELISA, FACS, FM, DB, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 Cy3
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应用备注
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Western blot (0.5-1 μg/mL): The antibody was tested using several His-tagged proteinsexpressed in insect cells and E. coli.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of > 0.5 mg/mL.
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缓冲液
- PBS, pH 7.4, without preservatives.
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized IgG is stable at 2-8 °C for one month from despatch and for one year when kept at -20 °C. The reconstituted antibody can be stored at 2-8 °C for one month or at -20 °C for one year.
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有效期
- 12 months
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- His Tag
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别名
- 6xHistidine Epitope Tag
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物质类
- Tag
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背景
- In the last couple of years many peptide sequences/epitopes for the purification of recombinant proteins have been established. These so-called
抗原
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