Periostin 抗体
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北京 101111
Quick Overview for Periostin 抗体 (ABIN115513)
抗原
See all Periostin (POSTN) 抗体适用
宿主
克隆类型
标记
应用范围
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序列
- MGHHHHHHHH HHSSGHIEGR HMRNNHYDKI LAHSRIRGRD QGPNVCALQQ ILGTKKKYFS TCKNWYKKSI CGQKTTVLYE CCPGYMRMEG MKGCPAVLPI DHVYGTLGIV GATTTQRYSD ASKLREEIEG KGSFTYFAPS NEAWDNLDSD IRRGLESNVN VELLNALHSH MINKRMLTKD LKNGMIIPSM YNNLGLFINH YPNGVVTVNC ARIIHGNQIA TNGVVHVIDR VLTQIGTSIQ DFIEAEDDLS SFRAAAITSD ILEALGRDGH FTLFAPTNEA FEKLPRGVLE RFMGDKVASE ALMKYHILNT LQCSESIMGG AVFETLEGNT IEIGCDGDSI TVNGIKMVNK KDIVTNNGVI HLIDQVLIPD SAKQVIELAG KQQTTFTDLV AQLGLASALR PDGEYTLLAP VNNAFSDDTL SMVQRLLKLI LQNHILKVKV GLNELYNGQI LETIGGKQLR VFVYRTAVCI ENSCMEKGSK QGRNGAIHIF REIIKPAEKS LHEKLKQDKR FSTFLSLLEA ADLKELLTQP GDWTLFVPTN DAFKGMTSEE KEILIRDKNA LQNIILYHLT PGVFIGKGFE PGVTNILKTT QGSKIFLKEV NDTLLVNELK SKESDIMTTN GVIHVVDKLL YPADTPVGND QLLEILNKLI KYIQIKFVRG STFKEIPVTV Y Remarks: The immunizati on antigen (75 kDa) is a protein containing 648 amino acids of recombinan t Human OSF-2 and 23 amino acids, N-Terminal HisTag and Xa - cleavage site (highlighte d). The antigen contains amino acid residues 22 to 669 of the Human OSF-2 precursor. The antigen includes all four fasciclin domains of OSF-2.
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特异性
- The antibody recognizes OSF-2.
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纯化方法
- Immunoaffinity Chromatography on Immoblized Recombinant Human OSF2
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免疫原
- Recombinant Human OSF-2 Source of Antigen: E. coli.
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应用备注
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ELISA. Western blot (0.1-1 μg/mL). Immunohistochemistry on Paraffin (1/100-1/5,000) and Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Add 0.05 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
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缓冲液
- 0.05 M Phosphate Buffer, 0.1 M NaCl, pH 7.2 without preservatives
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Stable the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Periostin (POSTN)
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别名
- Periostin
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背景
- Periostin is a disulfide linked 90 kDa 811 amno acid protein originally isolated as a osteoblast-specific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP)-2. OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.Synonyms: OSF-2, OSF2, Osteoblast-specific factor 2, PN, POSTN
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基因ID
- 10631
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NCBI登录号
- NP_001129406
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UniProt
- Q15063
抗原
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