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E2F1 抗体

This 小鼠 单克隆 antibody specifically detects E2F1 in WB, IF, IP 和 IHC (p). It exhibits reactivity toward 人 和 小鼠.
产品编号 ABIN115204
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for E2F1 抗体 (ABIN115204)

抗原

See all E2F1 抗体
E2F1 (E2F Transcription Factor 1 (E2F1))

适用

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  • 76
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  • 1
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人, 小鼠

宿主

  • 225
  • 11
  • 2
小鼠

克隆类型

  • 194
  • 44
单克隆

标记

  • 100
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  • 5
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  • 5
  • 5
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  • 2
  • 2
This E2F1 antibody is un-conjugated

应用范围

  • 202
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Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

KH95
  • 特异性

    This antibody reacts with a protein of 60 kDa.
    Cellular Localization: Nuclear.

    纯化方法

    Purified

    免疫原

    BALB/C mice were immunized with a recombinant Human E2F-1 protein.

    亚型

    IgG2a
  • 应用备注

    Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: 1/25-1/50 in an ABCmethod for 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Tonsil, Breast carcinoma.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 状态

    Liquid

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    E2F1 (E2F Transcription Factor 1 (E2F1))

    别名

    E2F1

    背景

    E2Fs are DNA-binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F-1 is proposed to be involved in several cellular processes that range from tumor suppressor, cell progression and oncogenesis. E2F-1 overexpression can also drive cells into apoptosis.Synonyms: E2F transcription factor 1, E2F-1, PBR3, PRB-binding protein E2F-1, RBBP3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, Transcription factor E2F1

    基因ID

    1869

    NCBI登录号

    NP_005216

    UniProt

    Q01094

    途径

    p53 Pathway, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy
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