E2F1 抗体
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北京 101111
Quick Overview for E2F1 抗体 (ABIN115204)
抗原
See all E2F1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
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This antibody reacts with a protein of 60 kDa.
Cellular Localization: Nuclear. -
纯化方法
- Purified
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免疫原
- BALB/C mice were immunized with a recombinant Human E2F-1 protein.
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亚型
- IgG2a
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应用备注
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Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: 1/25-1/50 in an ABCmethod for 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Tonsil, Breast carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- E2F1 (E2F Transcription Factor 1 (E2F1))
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别名
- E2F1
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背景
- E2Fs are DNA-binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F-1 is proposed to be involved in several cellular processes that range from tumor suppressor, cell progression and oncogenesis. E2F-1 overexpression can also drive cells into apoptosis.Synonyms: E2F transcription factor 1, E2F-1, PBR3, PRB-binding protein E2F-1, RBBP3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, Transcription factor E2F1
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基因ID
- 1869
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NCBI登录号
- NP_005216
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UniProt
- Q01094
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途径
- p53 Pathway, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy
抗原
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