CDC25A 抗体
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北京 101111
Quick Overview for CDC25A 抗体 (ABIN115127)
抗原
See all CDC25A 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
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This antibody reacts with a 65 kDa protein known as CDC25A.
Cellular Localization: Nuclear membrane. -
纯化方法
- Purified
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免疫原
- BALB/C mice were injected with purified recombinant CDC25A.
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亚型
- IgG2a
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应用备注
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Immunflourescence. Immunoprecipitation. Western Blot. Immunohistochemistry on Paraffin Embedded Sections: Use a dilution of 1/50-1/100 in an ABC method (30 minutes at room temperature, requireshigh temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior toimmunostaining)Recommended Positive Control: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))
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别名
- CDC25A
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背景
- The activity of cyclin-dependent kinases in cell cycle is regulated by the phosphorylation status, which is controlled by the antagonistic action of wee 1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B and CDC25C. CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1-S phase and G2 for CDC25B. CDC25C is mainly expressed in G2 phase.Synonyms: CDC-25A, Cell Division Cycle 25A, Dual specificity phosphatase Cdc25A, M-phase inducer phosphatase 1
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基因ID
- 993
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NCBI登录号
- NP_001780
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UniProt
- P30304
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途径
- Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase
抗原
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