Thrombospondin 1 抗体
Quick Overview for Thrombospondin 1 抗体 (ABIN115004)
抗原
See all Thrombospondin 1 (THBS1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
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This antibody is specific to 450 kDa protein (non-reduced) and 170 to 180 kDa (reduced) form of Thrombospondin.
Cellular Localization: Secretory granules, Golgi complex, endoplasmic reticulum -
纯化方法
- Purified
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免疫原
- BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.
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亚型
- IgG1
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应用备注
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Immunofluorescence. Immunoprecipitation. Western Blot. Immunohistochemistry on paraffin embedded sections: diluted of 1: 25-1: 50 in an ABC method (30 minutes at room temperature). Formalin fixedparaffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Tonsil
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Sodium azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Thrombospondin 1 (THBS1)
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别名
- Thrombospondin-1 (THBS1)
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背景
- Thrombospondin is a protein from platelet a granules. It is secreted at sites of platelet activation and aggregation and is involved in the differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.Synonyms: TSP, TSP1
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基因ID
- 7057
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NCBI登录号
- NP_003237
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UniProt
- P07996
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途径
- Autophagy
抗原
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