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Thrombospondin 1 抗体

The 小鼠 单克隆 anti-Thrombospondin 1 antibody is suitable to detect Thrombospondin 1 in samples from 哺乳动物. It has been validated for WB, IF 和 IHC (p).
产品编号 ABIN115004
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Thrombospondin 1 抗体 (ABIN115004)

抗原

See all Thrombospondin 1 (THBS1) 抗体
Thrombospondin 1 (THBS1)

适用

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哺乳动物

宿主

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小鼠

克隆类型

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单克隆

标记

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This Thrombospondin 1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

A6-1
  • 特异性

    This antibody is specific to 450 kDa protein (non-reduced) and 170 to 180 kDa (reduced) form of Thrombospondin.
    Cellular Localization: Secretory granules, Golgi complex, endoplasmic reticulum

    纯化方法

    Purified

    免疫原

    BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.

    亚型

    IgG1
  • 应用备注

    Immunofluorescence. Immunoprecipitation. Western Blot. Immunohistochemistry on paraffin embedded sections: diluted of 1: 25-1: 50 in an ABC method (30 minutes at room temperature). Formalin fixedparaffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Tonsil
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Sodium azide as preservative

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    Thrombospondin 1 (THBS1)

    别名

    Thrombospondin-1 (THBS1)

    背景

    Thrombospondin is a protein from platelet a granules. It is secreted at sites of platelet activation and aggregation and is involved in the differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.Synonyms: TSP, TSP1

    基因ID

    7057

    NCBI登录号

    NP_003237

    UniProt

    P07996

    途径

    Autophagy
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