This antibody detects PSMA expressed LNCap cell lines. Little or no cross-reactivity to benign prostate hyperplasia (BPH) or to normal prostatic tissue.
交叉反应 (详细)
Species reactivity (tested):Human.
纯化方法
Affinity Chromatography on Protein G.
免疫原
Crude membrane protein preparation from pooled prostate malignant carcinoma from China.
ELISA. Western blot. Immunofluorescence. Immunohistochemistry on Formalin-Fixed Paraffin Embedded and Frozen Tissue Sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore with double distillated water to adjust the final concentration to 1.0 mg/mL.
缓冲液
0.01 M PBS, pH 7.0 without preservatives.
储存液
Without preservative
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C/-80 °C
储存方法
Store lyophilized (preferably in a desiccator) at -20 °C and in aliquots at -80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time, it does not show decline in activity after two weeks at 4 °C.
PMSA (FOLH1) is a type II transmembrane glycoprotein belonging to the M28 peptidase family. Three functionally distinct proteins are encoded, including folylpoly-gamma-glutamate carboxypeptidase in the intestine, N-acetylated alpha-linked acidic dipeptidase 1 in the brain, and prostate-specific membrane antigen in the prostate. A mutation in the intestinal form may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. The form expressed in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. The prostate form is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants.Synonyms: FOLH, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, GCP2, Glutamate carboxypeptidase 2, Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, N-acetylated-alpha-linked acidic dipeptidase I, NAALAD1, NAALAdase, PSM, Prostate-specific membrane antigen, Pteroylpoly-gamma-glutamate carboxypeptidase