IL-8 抗体
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- 抗原 See all IL-8 (IL8) 抗体
- IL-8 (IL8) (Interleukin 8 (IL8))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This IL-8 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Neutralization (Neut), Enzyme Immunoassay (EIA)
- 纯化方法
- Affinity Chromatography on Protein G.
- 免疫原
- Purified recombinant human IL-8.
- 克隆位点
- I8-60
- 亚型
- IgG1
- Top Product
- Discover our top product IL8 Primary Antibody
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anti-Interleukin 8 (IL8) (AA 23-101) antibody
IL8 适用: 马 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 8 (IL8) (AA 21-99) antibodyIL8 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 8 (IL8) antibodyIL8 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
anti-Interleukin 8 (IL8) (AA 72-99) antibodyIL8 适用: 人 WB, IHC, ELISA, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 8 (IL8) antibodyIL8 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 8 (IL8) (AA 72-99), (C-Term) antibodyIL8 适用: 人 WB, FACS, IHC (p), IF 宿主: 兔 Polyclonal RB20676 unconjugated
anti-Interleukin 8 (IL8) (AA 23-101) antibodyIL8 适用: 犬 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 8 (IL8) (AA 23-101) antibodyIL8 适用: 犬 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C4 unconjugated
anti-Interleukin 8 (IL8) (AA 28-99) antibodyIL8 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 8 (IL8) (C-Term) antibodyIL8 适用: 人 WB, ELISA, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- ELISA: Can be used as a Tracer antibody in Sandwich ELISA for Detection of Human IL-8(matched pair with clone I8-S2, DM1022 as Capture antibody)Suggested Capture Coating Dose: 0.3 μg/well, substrate TMB. Neutralizing: In chemotaxis assay, the antibody inhibited 98.4 % of the chemotacticactivity of IL-8 on RB/293 cell using 5 μg/mL of MAb and 10 ng/mL of Human IL-8 in assay. This MAb inhibited the chemotactic effect of Human IL-8, had no inhibitory effect to MIP1-beta and RANTES in neutrophil chemotaxis assay. It indicates the MAb is specialneutralizing the IL-8 activity. The MAb has been shown the neutralizing activity on calciumion changes in human granulocytes using FACS. Western Blot: Concentration of 0.02-0.1 μg/mL MAb will allow visualization of 100 ng/laneof Human IL-8.
- 限制
- 仅限研究用
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- 溶解方式
- Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL.
- 缓冲液
- 0.01 M PBS, pH 7.0
- 储存液
- Without preservative
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody at 2-8 °C for one month or at -20 °C for longer.
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- 抗原
- IL-8 (IL8) (Interleukin 8 (IL8))
- 别名
- Interleukin-8 / IL8 (IL8 产品)
- 别名
- CXCL8 antibody, GCP-1 antibody, GCP1 antibody, LECT antibody, LUCT antibody, LYNAP antibody, MDNCF antibody, MONAP antibody, NAF antibody, NAP-1 antibody, NAP1 antibody, IL-8 antibody, AMCF-I antibody, il8 antibody, IL8 antibody, si:dkey-151b16.2 antibody, cxcli2 antibody, cxcl8 antibody, gcp-1 antibody, il-8 antibody, lynap antibody, mdncf antibody, monap antibody, nap-1 antibody, C-X-C motif chemokine ligand 8 antibody, interleukin 8 antibody, chemokine (C-X-C motif) ligand 8a antibody, chemokine (C-X-C motif) ligand 1-like antibody, chemokine (C-X-C motif) ligand 8 L homeolog antibody, CXCL8 antibody, il8 antibody, cxcl8a antibody, IL8 antibody, Cxcl8 antibody, LOC422654 antibody, cxcl8.L antibody
- 背景
- Interleukin 8 (IL-8), formerly called monocyte-derived neutrophil chemotactic factor, belongs to the C-X-C chemokine family. IL-8 has 4 cysteine residues, as do other members of the chemokine family, and the first two cysteine residues are separated by glutamine. IL-8 consists of 72 amino acids with a molecular weight of 8,000 daltons. IL-8 exhibits chemotactic activity in vitro for T cells, basophils and neutrophils. IL-8 activates neutrophils to release lysosomal enzymes including myeloperoxidase, -mannosidase and -glucuronidase. IL-1 induces the production of IL-8 from fibroblasts, keratinocytes, endothelial cells, hepatoma cells, astrocytoma cells, glioblastoma cells, lung epithelial cells, synovial membrane cells, melanocytes, melanoma cells and gastric cancer cells. Lipopolysaccharides will stimulate IL-8 production in monocytes/macrophages, and expressed on endothelial cells in response to inflammation.Synonyms: CXCL8, Emoctakin, GCP1, Granulocyte chemotactic protein 1, MDNCF, MONAP, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, NAP1, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor
- 基因ID
- 9606
- UniProt
- P10145
- 途径
- TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy
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