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EGF 抗体

EGF 适用: 人 ELISA (Capture) 宿主: 小鼠 Monoclonal S-134 unconjugated
产品编号 ABIN114522
发货至: 中国
  • 抗原 See all EGF 抗体
    EGF (Epidermal Growth Factor (EGF))
    适用
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    宿主
    • 210
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    小鼠
    克隆类型
    • 215
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    单克隆
    标记
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    This EGF antibody is un-conjugated
    应用范围
    • 200
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    • 53
    • 53
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    ELISA (Capture)
    特异性
    This antibody reacts with natural and recombinant human EGF. Does not cross react with other Human cytokines tested such as IL-1 beta, IL-8, MCAF, TGF-beta and SAA.
    交叉反应 (详细)
    Species reactivity (tested):Human.
    纯化方法
    Affinity Chromatograpghy on Protein G.
    免疫原
    Purified recombinant Human EGF, 6kDa, 53 amino acid residues
    克隆位点
    S-134
    亚型
    IgG1
    Top Product
    Discover our top product EGF Primary Antibody
  • 应用备注
    ELISA: This antibody can be used as a Capture antibody in sandwich ELISA applications forHuman EGF detection in combination with a monoclonal tracer/detection antibody. Suggested Capture Coating Dose: 0.3 μg/well, Substrate: TMB. If the above suggestedconditions are followed approximately 20 pg/mL of EGF in serum/plasma or 15 pg/mL ofEGF in cell medium/urine can be detected with an assay range of 0-1000 pg/mL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 溶解方式
    Restore with double distillated water is recommended to adjust the final concentration to 1.00 mg/mL
    浓度
    1.00 mg/mL
    缓冲液
    0.01 M PBS, pH 7.2 without preservatives
    储存液
    Without preservative
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Store the antibody at -20 °C. Can be shipped at 2 - 8 °C.
  • 抗原
    EGF (Epidermal Growth Factor (EGF))
    别名
    EGF (EGF 产品)
    别名
    HOMG4 antibody, URG antibody, AI790464 antibody, CEGF antibody, epidermal growth factor antibody, pro-epidermal growth factor antibody, EGF antibody, egf antibody, CpipJ_CPIJ020278 antibody, Egf antibody
    背景
    Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein. Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.Synonyms: Epidermal growth factor, HOMG4, Pro-epidermal growth factor, URG, Urogastrone
    基因ID
    1950
    NCBI登录号
    NP_001954
    UniProt
    P01133
    途径
    NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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