EGF 抗体
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Quick Overview for EGF 抗体 (ABIN114522)
抗原
See all EGF 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody reacts with natural and recombinant human EGF. Does not cross react with other Human cytokines tested such as IL-1 beta, IL-8, MCAF, TGF-beta and SAA.
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交叉反应 （详细）
- Species reactivity (tested):Human.
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纯化方法
- Affinity Chromatograpghy on Protein G.
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免疫原
- Purified recombinant Human EGF, 6kDa, 53 amino acid residues
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亚型
- IgG1
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anti-Epidermal Growth Factor (EGF) (AA 974-1026) antibody
EGF 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C11 unconjugated
anti-Epidermal Growth Factor (EGF) (AA 1000-1060) antibodyEGF 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor (EGF) antibodyEGF 适用: 人 WB, DB 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor (EGF) antibodyEGF 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 9D7F11 unconjugated
anti-Epidermal Growth Factor (EGF) (AA 690-720), (Center) antibodyEGF 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB16555 unconjugated
anti-Epidermal Growth Factor (EGF) (AA 609-751) antibodyEGF 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor (EGF) antibodyEGF 适用: 小鼠 WB, ELISA, Neut 宿主: 山羊 Polyclonal unconjugated
anti-Epidermal Growth Factor (EGF) antibodyEGF 适用: 人 ELISA, ICC 宿主: 小鼠 Monoclonal 4E11 unconjugated
anti-Epidermal Growth Factor (EGF) (AA 974-1026) antibodyEGF 适用: 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor (EGF) (AA 1-30) antibodyEGF 适用: 小鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: This antibody can be used as a Capture antibody in sandwich ELISA applications forHuman EGF detection in combination with a monoclonal tracer/detection antibody. Suggested Capture Coating Dose: 0.3 μg/well, Substrate: TMB. If the above suggestedconditions are followed approximately 20 pg/mL of EGF in serum/plasma or 15 pg/mL ofEGF in cell medium/urine can be detected with an assay range of 0-1000 pg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with double distillated water is recommended to adjust the final concentration to 1.00 mg/mL
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浓度
- 1.00 mg/mL
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缓冲液
- 0.01 M PBS, pH 7.2 without preservatives
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody at -20 °C. Can be shipped at 2 - 8 °C.
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- EGF (Epidermal Growth Factor (EGF))
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别名
- EGF
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背景
- Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein. Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.Synonyms: Epidermal growth factor, HOMG4, Pro-epidermal growth factor, URG, Urogastrone
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基因ID
- 1950
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NCBI登录号
- NP_001954
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UniProt
- P01133
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途径
- NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
抗原
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